相关实验视频

Updated: Jan 11, 2026

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

THRAISE:用于RNA-seq分析的自动化和可重复的网络平台

Hyeon Ho Heo¹, Soo-Jong Um¹

¹Department of Integrative Bioscience and Biotechnology, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, South Korea.

Molecules and cells

|November 15, 2025

概括

本研究介绍了一种精简的RNA测序 (RNA-seq) 分析网络平台,使复杂的生物信息学无需编码即可获得. 该系统提供从原始数据到功能丰富的全面分析,确保研究人员获得可重现的结果.

科学领域:

生物信息学是一种生物信息学.
计算生物学计算生物学
基因组学就是基因组学.

背景情况:

高通量RNA测序 (RNA-seq) 可以产生大量的数据.
复杂的生物信息管道往往需要专门的编程专业知识.
需要可访问的工具来民主化RNA-seq数据分析.

研究的目的:

为全面的RNA-seq分析提供简化,用户友好的网络平台.
让没有编程技能的研究人员能够进行复杂的RNA-seq分析.
提供可复制和可访问的RNA-seq数据解释.

主要方法:

开发一个网络平台,整合质量控制,调整和量化.
实现差异基因表达分析和功能丰富.
包含低复制数据集 (n=1) 的替代工作流程,使用每百万成绩单 (TPM).
使用基因本体学,基因和基因组的京都百科全书,反应体和基因组丰富分析进行功能丰富.

主要成果:

该平台通过完整的RNA-seq分析管道处理原始序列读取档案数据.
实时监控和交互式可视化增强了用户体验.
功能丰富分析是使用多个已建立的数据库进行的.
对于具有有限重复的数据集,可提供替代工作流.

结论:

整个转录组的高通量RNA-seq分析集成系统简化了复杂的RNA-seq分析.
它使所有技能水平的研究人员能够进行可重复的基因组研究.
该平台提高了RNA-seq数据解释的可访问性和效率.

关键词:

自动化自动化自动化自动化自动化通过RNA测序分析分析.全转录组高通量RNA测序分析集成系统综合系统.网络平台网络平台是一个网络平台.

更多相关视频

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...