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在没有长期质量控制的情况下提高代谢学数据的可比性,使用获得后校正策略
Elfried Salanon1, Blandine Comte1, Delphine Centeno1
1Université Clermont Auvergne, INRAE, UNH, Plateforme d'Exploration du Métabolisme, MetaboHUB Clermont, Clermont-Ferrand, France.
Analytica chimica acta
|November 15, 2025
概括
一项名为PARSEC的新策略通过标准化和过原始数据来提高代谢学数据的可比性. 这种方法增强了研究间的比较,并揭示了以前被分析变异性隐藏的生物学见解.
科学领域:
- 代谢学 代谢学 代谢学
- 生物信息学是一种生物信息学.
- 数据科学数据科学数据科学
背景情况:
- 代谢学分析技术的进步产生了高质量的数据.
- 在没有长期质量控制的情况下,整合代谢数据集受到纠正分析偏差的方法缺乏的阻碍.
- 这种局限性阻碍了跨研究的比较和代谢学在精密生物学中的影响.
研究的目的:
- 开发和评估获得后的战略,以提高代谢学数据的可比性.
- 为了克服阻碍研究相互比较的瓶.
- 通过改进数据互操作性,增强代谢学在精密生物学中的影响.
主要方法:
- 一个三步工作流程 (PARSEC) 涉及数据提取,标准化和功能过.
- 将PARSEC战略应用于两个案例研究.
- 将PARSEC与局部估计散射图平滑 (LOESS) 方法进行比较.
主要成果:
- 帕塞克战略有效地减少了群体间的变化,并改善了样本分布的均性.
- 在这两项案例研究中,数据可比性显著提高.
- 与LOESS方法相比,被变异性掩盖的生物信息被更清楚地揭示出来.
结论:
- 通过将批量标准化和混合建模相结合的PARSEC战略,提高了代谢学中的数据可比性和可扩展性.
- 这种方法最大限度地减少了分析条件的影响,同时通过解决批量和组效应来保持生物变异性.
- PARSEC提供了一种有价值的工具,可以在缺乏共同质量控制样本的研究或队列中协调数据集.
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