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人类呼吸道上皮细胞中WUPyV的细胞病变效应和形态发生

Yiman Huang^1,2, Chao Wang^1,2, Aijun Chen^1,2

¹National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.

Journal of medical virology

|November 16, 2025

概括

华盛顿大学多细胞瘤病毒 (WUPyV) 感染人体呼吸道,并通过克拉斯林介导的内细胞分解进入细胞. 这项研究揭示了WUPyVV.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
呼吸系统医学呼吸系统医学

背景情况:

华盛顿大学多发性瘤病毒 (WUPyV) 在人类中普遍存在,但由于文化挑战,其致病性尚不清楚.
了解WUPyV感染机制对于评估其作为呼吸道病原体的作用至关重要.

研究的目的:

研究WUPyV在人类呼吸道上皮的传染性内细胞化,复制和病原机制.
使用人类呼吸道新型器官模型,对WUPyV的病毒形态变异和细胞热带性进行鉴定.

主要方法:

使用人类呼吸道器官 (HAO) 开发一种新的感染模型.
描述了WUPyV复制动力学,细胞热带,内细胞化途径和人类呼吸道上皮质 (HAE) 的形态发生.
显微镜分析观察病毒运输,组装和释放.

主要成果:

WUPyV有效地复制和感染呼吸道上皮层内的多种细胞类型.
WUPyV利用克拉特林介导的内细胞酶酶进入细胞,独立于其他途径.
病毒复制发生在细胞核中,通过囊泡和分裂细胞进行组装和细胞外释放.
高位数的WUPyV感染会对HAE和眼造成显著的结构损伤.

结论:

WUPyV采用特定的内细胞通路和核复制策略.
该病毒对呼吸道上皮造成了相当大的超结构性损伤.
WUPyV是一种被低估的人类病原体,在呼吸道中具有显著的病原性潜力.

关键词:

在WU中,多重病毒.细胞内症是指内细胞内症.人类呼吸道上皮质形态发生 (morphogenesis) 是一种形态的产生.

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