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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
Next-generation Sequencing03:00

Next-generation Sequencing

97.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.5K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.5K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

4.0K
4.0K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.5K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.5K

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相关实验视频

Updated: Jan 6, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

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识别和可视化通过Ribo-Seq,转录组合和ggRibo的新型小型开放阅读框架 (sORFs).

Hsin-Yen Larry Wu1, Isaiah D Kaufman1, Polly Yingshan Hsu2

  • 1Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI, USA.

Methods in molecular biology (Clifton, N.J.)
|November 16, 2025
PubMed
概括

我们开发了一种生物信息学管道,使用Ribo-seq数据找到和可视化小说翻译小开放阅读框架 (sORFs). 这种工具有助于揭示隐藏的sORF及其在基因调节中的功能.

关键词:
里博-seqq 的意思里博塔佩尔 (RiboTaper) 是一种核糖体造型分析 核糖体造型分析小型的ORFs是一个ORF.转录组组件组件ggRiboRibo是什么意思

更多相关视频

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
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相关实验视频

Last Updated: Jan 6, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
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Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 小开放的读取框架 (sORFs) 对于细胞调节至关重要,但在基因组注释中经常被遗漏.
  • sORFs的转化活性与非编码RNA生物发生和信号通路有关.
  • 由于它们的小尺寸和缺乏正典特征,准确识别翻译的sORF是具有挑战性的.

研究的目的:

  • 提出一种新的生物信息管道,用于从Ribo-seq数据中识别和可视化翻译的sORF.
  • 为了能够在Arabidopsis中发现以前未被注释的sORFs.
  • 为新型sORFs提供一个功能性特征的框架.

主要方法:

  • 整合Ribo-seq/RNA-seq预处理和映射.
  • 可选的转录组组件用于检测未注释的转录.
  • 使用RiboTaper发现ORF,并使用ggRibo基因查看器进行可视化.

主要成果:

  • 为Ribo-seq数据分析建立了一个全面的管道.
  • 该管道有助于发现和高分辨率可视化翻译的sORFs.
  • 在阿拉比多普西斯 (Arabidopsis) 发现隐藏的sORF的实用性已被证明.

结论:

  • 开发的管道为系统的sORF识别和可视化提供了一个强大的方法.
  • 这种方法有助于发现和未来对新型sORFs的功能研究.
  • 该管道可适应在各种生物体中进行sORF发现.