干扰可转移的元素:ISXoo15转移酶作为对抗Xanthomonas oryzae pv.的第一类抗菌标. 一个叫做"Oryzae"的
Funeng Lu1, Ting Liu1, Tangbing Yang1
1State Key Laboratory of Green Pesticide, Center for R&D of Fine Chemicals, Guizhou University, Guiyang, China.
Molecular plant pathology
|November 17, 2025
概括
研究人员发现ISXoo15转化酶是控制大米中的细菌叶病的新目标. 这种新化合物J9有效地抑制了Xanthomonas oryzae pv. oryzae (Xoo) 通过准这种转基因酶.
科学领域:
- 植物病理学 植物病理学
- 细菌学 细菌学是一门学科.
- 药物发现 药物发现 药物发现
背景情况:
- 植物病原性细菌由于广泛的耐药性和缺乏新目标,因此带来了重大挑战.
- 可转移的元素是毒性的关键驱动因素,但作为治疗点,它们仍然在很大程度上未被探索.
- 克桑托莫纳斯里扎 (Xanthomonas oryzae pv.) 是一个植物. 桃菌 (Xoo) 致使大米的细菌叶病破坏性.
研究的目的:
- 为了确定控制植物病原菌的新型分子标.
- 调查在Xoo.中针对可转换元素的潜力.
- 评估一种新型的胺基替代性流素衍生物 (J9) 作为抗菌剂.
主要方法:
- 在体外和体内测试以评估J9对Xoo的疗效.
- 光亲和探针合成 (P-J9) 和基于活动的蛋白质概况,以确定J9的目标.
- 鉴定目标 (PXO_03433/ISXoo15转化酶) 的基因淘汰和补充研究.
- 反转录定量PCR和蛋白质基因分析分析基因表达和途径变化.
主要成果:
- 与商业药物相比,J9在体外对Xoo (EC50 = 0.12 mg/L) 显示出更高的活性.
- 在体内,J9对抗大米细菌叶病的治疗和保护效果有所提高.
- ISXoo15转体酶 (编码为PXO_03433) 被确定为J9.9的特定杀菌标.
- 破坏PXO_03433减弱了Xoo的毒性,而J9治疗降低了其表达和相关的DNA修复途径.
结论:
- ISXoo15转体酶是针对植物病原菌细菌的新型抗菌策略的可用药物标.
- J9代表了一种有前途的新疗法剂,用于控制大米细菌叶病.
- 准细菌转体酶提供了一种先进的方法来对抗植物病原体.
相关概念视频
Transposons
1.3K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
1.3K
Overview of Transposition and Recombination
18.7K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
18.7K
DNA-only Transposons
17.1K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
17.1K
piRNA - Piwi-interacting RNAs
7.5K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.5K
Transgenic Organisms
33.1K
Overview
33.1K
Transgenic Plants
8.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.4K


