在真菌中同质二烯生物合成
Lin Zhou1, Tatjana Reuter1, Kiara Schumann1
1Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78464, Konstanz, Germany.
Angewandte Chemie (International ed. in English)
|November 17, 2025
概括
科学家们发现了第一个真菌同烯通路,挑战了异烯规则. 这一发现揭示了新的自然产品和对烯生物合成的进化见解.
科学领域:
- 自然产品化学 自然产品化学
- 生物化学 生化学
- 菌类学 菌类学是指菌类学.
背景情况:
- 同烯,天然产品与甲基化骨干,挑战传统的异烯规则.
- 对同烯的生物合成途径在细菌中已知,但在真菌中以前没有.
- 生物性异烯规则认为烯由C5单元组成.
研究的目的:
- 为了发现真菌王国中第一个同烯生物合成途径.
- 为了研究参与真菌同烯生产的酶.
- 为了了解真菌同基生物合成的进化起源.
主要方法:
- 在真菌中进行有针对性的基因组数据挖掘.
- 在体外复制已识别的生物合成途径.
- 甲基转移酶 (NdiMT) 和旋酶 (NdiTC) 酶的功能分析.
- 对基因起源的遗传学分析.
主要成果:
- 在Neonectria ditissima*中发现了第一个真菌同基生物合成途径.
- 鉴定了一种新型的同质二烯与一个heptamethylbicyclo[3.3.1]nonane支架.
- 从细菌到真菌的甲基转移酶的水平基因转移的证据.
- 这表明烯环酶是从真菌祖先进化而来的.
结论:
- 菌类拥有生产同烯的途径,扩大它们已知的生物合成能力.
- 发现了一种新的甲基化烯支架,提供了新的化学多样性.
- 横向基因转移在真菌中新型自然产品途径的演变中发挥着作用.
- 这些发现促使进一步研究甲基化的进化和功能意义.
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