Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones01:15

NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones

In aldehydes, the hydrogen atom connected to the carbonyl carbon helps distinguish aldehydes from other carbonyl compounds using ¹H NMR spectroscopy. The closeness of aldehydic hydrogen to the electrophilic carbonyl carbon highly deshields the hydrogen atom causing its signal to appear around 10 ppm in the ¹H NMR spectra. α hydrogens split the aldehydic proton signal, which helps identify the number of α hydrogens in the molecule. For instance, one α hydrogen creates a doublet for an aldehydic...
NMR and Mass Spectroscopy of Carboxylic Acids01:30

NMR and Mass Spectroscopy of Carboxylic Acids

In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the disappearance of the acidic...
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The discovery and development of small molecule immune potentiators (SMIPs) as next-generation vaccine adjuvants, the creation of AS37.

Current opinion in virology·2026
Same author

Multimodal neuromonitoring in neonatal hypoxic-ischemic encephalopathy and the association with neurodevelopmental outcomes: A Multicenter Study.

The Journal of pediatrics·2026
Same author

Trending ability of electrical cardiometry for non-invasive cardiac output monitoring in preterm neonates during the transitional period: a polar plot analysis.

European journal of pediatrics·2026
Same author

FASN for diffuse malignant peritoneal mesothelioma: a prognostic biomarker after CRS+HIPEC and a therapeutic target.

Journal of translational medicine·2026
Same author

Controlling Macrophage Uptake of Gold Nanoparticles through the Design of an Effective Heterogeneous Coating.

ACS applied materials & interfaces·2026
Same author

Human Transthyretin with a Tailored Gd(III) Complex as a High-Relaxivity MRI Contrast Agent.

Inorganic chemistry·2026

相关实验视频

Updated: Jun 30, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
08:54

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

Published on: May 1, 2017

26.8K

在使用NMR的药物成分中快速而简单的脂质量测量.

Francesco Currò1,2,3, Ander Eguskiza3, Linda Cerofolini1,2,4

  • 1Department of Chemistry Ugo Schiff (DICUS), University of Florence, via Della Lastruccia 3, 50019 Sesto Fiorentino, Italy.

ACS omega
|November 17, 2025
PubMed
概括

核磁共振 (NMR) 光谱学,特别是PULCON方法,可以准确量化脂质体中的脂质. 这种快速的NMR技术非常适合制药制造业的质量控制.

更多相关视频

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

15.1K
Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.6K

相关实验视频

Last Updated: Jun 30, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
08:54

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

Published on: May 1, 2017

26.8K
A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
09:32

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy

Published on: January 30, 2019

15.1K
Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.6K

科学领域:

  • 制药科学 制药科学
  • 分析化学 分析化学
  • 材料科学 材料科学 材料科学

背景情况:

  • 脂质体是药品中至关重要的纳米级输送系统.
  • 精确的脂质量测量对于脂质体配方的质量和有效性至关重要.
  • 现有的方法可能缺乏大规模制造所需的速度和一致性.

研究的目的:

  • 评估核磁共振 (NMR) 光谱法,以精确量化脂质在脂质体.
  • 评估PULCON (基于脉冲长度的度确定) 方法用于分析常见的脂质体脂质.
  • 为了比较NMR量化与超高性能液态染色学与蒸发光散射检测 (UPLC-ELSD).

主要方法:

  • 使用NMR光谱与内部参考和外部标准PULCON方法量化脂质.
  • 脂质的分析包括DSPC,DOPC,胆固醇和DMPE-PEG2K.
  • 用UPLC-ELSD对脂质体溶液进行比较分析.

主要成果:

  • 内部和外部标准的PULCON NMR方法都显示出高准确性,精度和可重复性.
  • 普尔康核磁共振技术提供了更高的一致性和更快的分析时间.
  • 核磁共振量化结果与通过UPLC-ELSD获得的结果相似.

结论:

  • 普尔康NMR光谱是一种可靠和高效的方法,用于脂质体中的脂质量定量.
  • 普尔康NMR的速度和准确性使其适用于工业质量控制和配方优化.
  • 这项研究支持开发用于制药制造的快速,准确的脂质量定量.