分子和表达分析表明,融合转录在调解豆中的非生物应激反应中的作用
Fiza Hamid1, Shafaque Zahra2, Shailesh Kumar1
1Bioinformatics Lab, BRIC-National Institute of Plant Genome Research, New Delhi, India.
Frontiers in plant science
|November 17, 2025
概括
这项研究在豆中确定了328个融合转录 (FT),其中大多数是通过转接生成的. 这些FT可以增强功能多样性,并在非生物应激反应中发挥作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 文字转录学 (Transcriptomics) 是一个学科.
- 基因组学就是基因组学.
背景情况:
- 转录组多样性是理解基因调节的关键.
- 融合转录 (FT) 产生于基因融合或RNA剪接,并使用高通量测序检测到.
- 在小 (Cicer arietinum) 中研究FT可以揭示新的调节机制.
研究的目的:
- 识别和描述豆的融合转录在不同的组织和非生物应激条件中.
- 探索已识别的FT的功能影响和保护.
- 研究FT在豆对干旱和盐度压力的反应中的作用.
主要方法:
- 高通量RNA-Seq分析五个小豆组织 (茎,叶子,芽,花,).
- 在干旱和盐度压力条件下对转录组的分析.
- 用RT-PCR和桑格测序进行FT验证.
- 生物信息分析包括功能注释,丰富和跨基因型的保护分析.
主要成果:
- 在小中发现了328个独特的FT,其中69%显示了正规拼接部位,这表明了跨拼接.
- 功能注释表示FT可能会扩大功能多样性.
- 10个FT得到了验证,代表了豆中首次描述的FT.
- 表达式分析揭示了跨组织和压力条件的FT的上下文依赖调节.
- 在17种小基因型中保存了120个融合基因对.
结论:
- 融合转录是豆转录组的重要组成部分.
- FT可能有助于功能多样性和监管机制,特别是在对非生物压力的反应中.
- 已识别和验证的FT为进一步研究小基因调节和应激适应提供了基础.
更多相关视频
10:29Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
6.9K
10:10Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Published on: May 29, 2010
16.2K
相关概念视频
Transcription
154.9K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
154.9K
Alternative RNA Splicing
24.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.6K
Responses to Heat and Cold Stress
14.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.6K
Responses to Salt Stress
14.4K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.4K
What is Gene Expression?
10.9K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
10.9K
