阶段特异性GnRH给药对卵巢功能标志物,排卵时间和雌性同步母羊的生育能力的影响
Jose Francisco Cox1, Felipe Navarrete1, Antonio Bocic1
1Faculty of Veterinary Sciences, Universidad de Concepción, Chillán, Chile.
Frontiers in veterinary science
|November 17, 2025
概括
在卵泡期晚期服用GnRH可以改善羊的排卵时间和生育能力. 早期GnRH在绵羊协议中的使用会改变卵泡大小,但不会改变生殖成功.
科学领域:
- 兽医科学 兽医科学 兽医科学
- 生殖生物学 生殖生物学
- 动物科学动物科学
背景情况:
- 在羊群中,低于最佳的生育率限制了雌性同步 (ES) 协议.
- 释放性激素 (GnRH) 可能通过控制排卵时间或促进卵泡均性来改善卵巢结果.
研究的目的:
- 测试GnRH给药是否能改善经受雌性同步治疗的母羊的卵巢功能和生育能力.
- 评估GnRH定时对排卵控制,卵泡发育和生育结果的影响.
主要方法:
- 三项实验使用基于CIDR的协议进行,包括或不包括eCG和GnRH,以及Synchrovine协议.
- 与排卵相比,绵羊在不同阶段 (晚期卵泡阶段和早期卵泡阶段) 用GnRH治疗.
- 在自然交配后评估了卵巢形态,内分泌标志物和生育能力 (怀孕,羔羊,生育能力).
主要成果:
- 在CIDR去除后36小时服用GnRH缩短了排卵间隔和集中排卵时间 (实验1).
- 晚期卵泡期的GnRH治疗显著增加了商业绵羊的生育率 (实验2).
- 早期卵泡期的GnRH给药减少了卵泡直径的异质性,但没有影响排卵或生育能力 (实验3).
结论:
- 在较晚的卵泡阶段给予GnRH可增强排卵的同步性,并改善绵羊的生育能力.
- 早期使用GnRH用于卵泡均化并未转化为改善生殖结果.
- 在雌性同步协议中优化GnRH时间对于最大限度地提高绵羊的生育能力至关重要.
更多相关视频
09:01Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
Published on: September 15, 2012
119.5K
09:07Protocol for Studying Extinction of Conditioned Fear in Naturally Cycling Female Rats
Published on: February 23, 2015
13.9K
相关概念视频
Ovarian Cycle
3.4K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
3.4K
Hormonal Control of the Ovarian Cycle
6.4K
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
6.4K
Hormonal Regulation of the Menstrual Cycle
1.4K
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
1.4K
Proliferative Phase
1.2K
The proliferative phase typically occurs after menstruation and lasts between 6 to 13 days in a standard 28-day cycle. This phase involves the reconstruction of the endometrium, guided by estrogen produced by the developing ovarian follicle.
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
1.2K
Oogenesis
3.7K
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
3.7K
