鲁比斯科慢慢地穿过生命之树
Benoit de Pins1, Cyril Malbranke2,3, Jagoda Jabłońska1
1Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.
概括
研究人员探索了来自各种细菌和古生物的250多种鲁比斯科酶. 他们发现,这种关键的碳固定酶通常很慢,其速度很少超过每秒30个反应.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 进化生物学 进化生物学
背景情况:
- 鲁比斯科对碳固定至关重要,作为无机碳进入生物圈的主要酶.
- 之前的动力学研究集中在真核体鲁比斯科上,限制了对酶的充分多样性和速度的理解.
- 已知最快的鲁比斯科变体先前在原核生物中被发现,这表明更广泛的多样性可能拥有更快的酶.
研究的目的:
- 描述来自细菌和古生物的大量多样化的鲁比斯科酶.
- 评估rubisco在广泛的基因组学范围内的催化速率.
- 为了研究与更快的鲁比斯科碳氧化率相关的进化背景.
主要方法:
- 来自各种细菌和古生物学物种的250多种鲁比斯科酶的表征.
- 在和CO2度下测量碳素化速率.
- 机器学习模型的应用,用于预测~68000个序列化的鲁比斯科变体的速率.
主要成果:
- 鲁比斯科被证实是生命树上的相对较慢的酶,在30°C的速度不超过每秒大约30个反应.
- 较快的鲁比斯科亚类与特定的进化背景有关,例如微氧环境或二氧化碳度机制.
- 创建了一个关于天然鲁比斯科变种及其率的综合数据集,作为未来研究的基准.
结论:
- 这项研究扩大了已知的鲁比斯科酶的多样性,翻了一番以前的覆盖范围.
- 鲁比斯科的催化速率在整个生命中通常受到限制,尽管与环境因素相关的变化.
- 开发的数据集和预测模型为理解和设计Rubisco的催化效率提供了基础.
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