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大脑衍生的神经营养因子通过斑马鱼的TrkB-MAPK-PI3K通路促进卵细胞成熟

Chinelo Uju¹, Suraj Unniappan¹

¹Laboratory of Integrative Neuroendocrinology, Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5B4, Canada.

Molecular and cellular endocrinology

|November 17, 2025

概括

大脑衍生神经营养因子 (BDNF) 通过促进小胞生成和卵细胞成熟来增强斑马鱼的繁殖能力. 这些效应通过TrkB信号通路进行介导,涉及MAPK,PI3K和PLC-γ,这对雌性斑马鱼的繁殖成功至关重要.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
神经营养素信号传递

背景情况:

已知来自大脑的神经营养因子 (BDNF) 对斑马鱼的繁殖有积极的影响.
通过BDNF发挥其促生殖作用的精确分子机制在很大程度上是未知的.
假设TrkB信号传递在雌性斑马鱼中调解BDNF的生殖功能.

研究的目的:

为了阐明参与斑马鱼中BDNF介导的繁殖的信号通路.
调查TrkB信号传递在BDNF对生生成和卵细胞成熟的影响中的作用.
识别斑马鱼繁殖过程中受到BDNF影响的下游分子标和途径.

主要方法:

使用斑马鱼肝 (ZFL) 细胞和斑马鱼毛囊进行实验分析.
采用了一种TrkB对抗剂 (ANA-12) 和MAPK,PI3K和PLC-γ信号通路的抑制剂.
评估了关键生殖基因 (例如,维特洛根因,sf-1) 和蛋白质水平的转录丰度.
检查了卵细胞成熟和相关基因和酶的表达.

主要成果:

在ZFL细胞中,BDNF上调调节了维特洛根因和类固醇原因子1的转录,这种效应被TrkB抑制阻断.
MAPK,PI3K和PLC-γ通路对于BDNF诱导的生和蛋白质生产至关重要.
BDNF增强了卵细胞成熟和与成熟相关的基因的表达,由TrkB,MAPK和PI3K信号介导.

结论:

在斑马鱼中,BDNF通过TrkB-MAPK/PI3K/PLC-γ信号级联促进了生命生成.
在斑马鱼中,BDNF通过TrkB-MAPK/PI3K通路增强卵细胞成熟.
这些发现澄清了BDNF在调节斑马鱼繁殖中的作用背后的分子机制.

关键词:

鱼类鱼类鱼类鱼类毛囊毛囊是一种毛囊.增长因素增长因素激素这些激素.卵巢卵巢卵巢生殖生殖的繁殖

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