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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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天然深度美容溶剂可实现可编程的无冷却冷保存.

Hengxin Han1,2,3, Ying Wang1,2,3, Taijie Zhan1,2,3

  • 1Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology, Shanghai 200093, China.

Langmuir : the ACS journal of surfaces and colloids
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概括

像PS21和PS31这样的天然深层水溶剂 (NADES) 显示出 cryoprotectants 的前景,与生物银行应用中的 DMSO 相比,显著改善细胞活力和减少冰损伤.

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科学领域:

  • 低温生物学 低温生物学
  • 生物材料科学 生物材料科学
  • 可持续化学 可持续化学

背景情况:

  • 低温保存对于生物样本的长期储存至关重要.
  • 传统的冷保护剂,如二甲基硫氧化物 (DMSO),可以表现出毒性.
  • 需要有效的低毒性冷保护剂来改善生物银行.

研究的目的:

  • 评价天然深层环氧溶剂 (NADES),特别是PS21和PS31,作为新型冷保护剂.
  • 评估这些NADES的物理化学特性和冷保护功效.
  • 为了比较NADES与DMSO等传统冷保护剂的性能.

主要方法:

  • 使用FTIR,DSC和冷显微镜进行物理化学表征.
  • 评估细胞活力 (A549细胞) 和解后恢复的生物测试.
  • 优化冷却协议以确定最佳的冷保存参数.

主要成果:

  • PS21和PS31显著抑制了冰的结晶,并减少了再结晶损伤.
  • 与DMSO相比,NADES显示出更高的未水含量.
  • 与DMSO相比,NADES的细胞活力和增殖率明显高于DMSO,特别是在优化的冷却协议下.

结论:

  • NADES (PS21和PS31) 是一种有效的低毒性冷保护剂.
  • 这些溶剂为生物银行提供了一个可持续和可扩展的替代方案.
  • NADES提高了样品质量,并简化了冷保存工作流程.