翻译协因子PABP相互作用蛋白11月光作为转录激活剂调节质合成基因表达
Yingchun Wan1, Xiaoqing Liu1, Ye Tang1
1Rice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
The Plant journal : for cell and molecular biology
|November 18, 2025
概括
米的男性生育能力依赖于精确的基因调节. 一种翻译因子OsPAIP11通过控制糖合成基因转录和潜在的翻译来影响花粉发育.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生殖生物学 生殖生物学
背景情况:
- 米的男性生育能力对作物产量至关重要,并由复杂的遗传网络来调节.
- 与PABP相互作用的蛋白质在翻译中起作用,但它们在植物繁殖发育中的特定功能尚未完全理解.
研究的目的:
- 克隆和描述OsPAIP11的功能,一种新的PABP相互作用蛋白,在米男性生育.
- 阐明OsPAIP11影响花粉发育和男性生育能力的分子机制.
主要方法:
- 基因克隆和突变分析 (ospaip11).
- 亚细胞局部化和蛋白质域分析 (RRM2域).
- 转录活性测定和染色体免疫沉降 (ChIP) 测定目标基因 (OsGSL5,OsGAMYB).
主要成果:
- 在ospaip11突变中显示了合成, tapetum 亡和花粉壁形成的缺陷,类似于dcet1突变.
- OsPAIP11局部化到细胞质和细胞核,其RRM2域表现出转录激活.
- OsPAIP11直接调节合成基因OsGSL5和OsGAMYB的转录,影响花粉的发育.
结论:
- OsPAIP11是大米男性生育能力的关键调节者,主要通过对质合成基因的转录控制.
- OsPAIP11在转录中的双重作用和在翻译中的潜在参与凸显了它对男性性生殖的复杂贡献.
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