无催化剂原纤维交叉连接用于工程无异性质和机械坚固的组织支架
JuYeon Kim1, Hanjun Hwangbo1, ByungJoon Choi1
1Department of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 18, 2025
概括
研究人员使用一种新的生物直角交叉连接方法开发了增强的原体水凝. 这些机械强,对齐的支架支持干细胞生长,并在体内促进功能性肌肉再生.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 从像原蛋白这样的天然蛋白质中开发机械弹性水凝对于组织再生至关重要.
- 在这些水凝中同时实现细胞兼容性和结构完整性是一个重大挑战.
研究的目的:
- 通过使用生物直角交叉连接策略,设计机械增强,对齐的原体基.
- 评估这些支架在促进功能性肌肉再生方面的细胞兼容性和有效性.
主要方法:
- 通过与罗达胺和聚乙烯糖醇 (PEG) 的生物直角交联制造密集的原蛋白水凝.
- 交叉连接与湿的整合,以产生单轴对齐的原纤维.
- 在对齐的细丝中封装人类脂肪衍生的干细胞 (hASCs),并评估机械传导信号.
主要成果:
- 生物直角交叉连接方法提高了水凝的刚性和机械强度.
- 对齐的原纤维支持hASC封装并激活机械传导信号,包括细胞骨组织和肌性基因表达.
- 在小鼠体积肌肉损失模型中,带有细胞的细丝促进了体外分化和体外功能肌肉再生.
结论:
- 开发的战略提供了一个可扩展的,细胞兼容的平台,用于创建对齐的,基于蛋白质的支架,具有可调节的属性.
- 这种方法推进了再生医学的工具包,特别是对于需要机械坚固和生物活性组织结构的应用.
相关概念视频
The Structure of Intermediate Filaments
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm). These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate filaments...
Intermediate filaments...
Formation of Intermediate Filaments
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.
Cell-matrix's Response to Mechanical Forces
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
Fibrous Proteins
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...
Fiber Reinforced Concrete
Fiber-reinforced concrete significantly enhances the structural and nonstructural properties of traditional concrete by incorporating fibers like steel, glass, and polymers. These fibers, varying from natural ones such as sisal and cellulose to manufactured ones like polypropylene and Kevlar, are mixed into hydraulic cement with aggregates. Steel fibers, often preferred for their robustness, contribute to improved ductility, toughness, and post-cracking performance. The concrete is classified...
Ferrocement
Ferro-cement is a distinctive construction material that represents an innovative variant of reinforced concrete, characterized by its unique composition and the method by which it is formed. Unlike standard reinforced concrete, which relies on larger steel bars for reinforcement, ferro-cement utilizes densely packed layers of mesh or fine rods, fully encased in cement mortar. This composition allows for the creation of structures that are significantly thinner and more flexible than their...


