相关实验视频

Updated: Jan 11, 2026

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing

Published on: January 20, 2023

mRNA-LNP疫苗:合理的设计,优化输送和临床翻译

Sangni Jiang^1,2, Zhihui Lu^1,2

¹College of Chemical and Biological Engineering Zhejiang University Hangzhou, Zhejiang 310058, China. 0623958@zju.edu.cn.

Journal of materials chemistry. B

|November 18, 2025

概括

通过脂质纳米颗粒 (LNP) 传递的信使RNA (mRNA) 疫苗显示出希望,但面临着传递和免疫性挑战. 优化mRNA序列,LNP配方和表面功能化是下一代疫苗和精密医学的关键.

科学领域:

生物技术和制药科学生物技术和制药科学
疫苗学疫苗学疫苗学
纳米医学是一种纳米医学.

背景情况:

使者RNA (mRNA) 疫苗因传递效率和免疫性低而受到阻碍,限制了它们在传染病预防和癌症治疗中的使用.
脂质纳米颗粒 (LNP) 是mRNA的主要非病毒传递系统,保护有效载荷,增强细胞吸收,并促进内体逃生.
COVID-19 mRNA-LNP疫苗的成功凸显了快速设计和灵活生产的优势.

研究的目的:

系统地审查mRNA-LNP疫苗的开发.
分析mRNA序列,LNP配方和表面功能化的核心优化策略.
探索未来的方向,以克服临床翻译瓶.

主要方法:

分析mRNA序列工程,包括核酸酶修饰和UTR/多A尾部优化.
审查LNP制定策略,如组件比率优化和SPOT策略.
使用小分子,和抗体检查LNP表面功能化技术.

主要成果:

优化策略提高mRNA稳定性,翻译效率,免疫反应调制和器官向.
针对传染病和癌症的候选mRNA-LNP疫苗已经进入临床试验.
目前的局限性包括目标准确度不足,潜在的免疫性/毒性以及普遍的交付挑战.

结论:

未来的mRNA-LNP疫苗开发需要多学科的创新,专注于可降解脂质,新型向配体和生物相容聚合物.
人工智能 (AI) 可以加速LNP配方设计和性能预测.
mRNA-LNP技术有可能成为下一代精密医学的基石.

更多相关视频

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

相关概念视频

Microorganisms in Medicine and Therapeutics

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...