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模拟胃肠道消化平台的比较,复杂度和样本质量要求各不相同,使用普通豆 (Phaseolus vulgaris L.) 作为模型

Tayah M Bolt¹, Margaret Riggs¹, Weiyi Sun²

¹Department of Plant Sciences, University of California, Davis, Davis, CA, USA.

Journal of the science of food and agriculture

|November 18, 2025

概括

模拟的消化模型准确地测量了普通豆中的营养物质生物可访问性. 动态模型,即使是简单的模型,在评估粉,蛋白质和化合物方面的性能优于静态模型,有助于植物育种和粮食开发.

科学领域:

食品科学食品科学食品科学
营养科学营养科学
农业科学农业科学

背景情况:

评估植物性食品中的营养物质生物可访问性需要生理上相关的消化模型.
目前的方法通常受样本大小和吞吐量限制,阻碍了植物育种和食品开发.
模拟的消化模型为测量营养素的生物可用性提供了一个解决方案.

研究的目的:

开发和比较四个模拟的胃消化模型 (三个动态,一个静态) 用于小样本质量.
在不同的生长条件下评估常见豆 (Phaseolus vulgaris L.) 基因型中的营养物质生物可访问性.
评估动态消化模型对于高通量分析的实用性.

主要方法:

开发并测试了四种体外消化模型:三种动态 (模拟静止,分泌,流动) 和一种静态.
配对的胃模型与静态小肠阶段.
在四个普通豆样本组中分析了可生物利用的粉,蛋白质,总和抗氧化能力.

主要成果:

更复杂的动态消化模型产生了更高的可生物利用的营养水平 (粉和蛋白质水解,,抗氧化能力).
即使是简单的动态模型,与静态模型相比,也显示出优异的结果.
在豆类基因型之间的蛋白质水解以及在生长环境之间的和蛋白质水解中观察到显著差异.

结论:

规模较小的动态消化模型对营养物质生物可访问性的高通量分析具有前景.
这些模型为植物育种和食品产品开发提供了宝贵的见解.
模拟消化模型是测试植物性食品中的生物可访问营养素的有效工具.

关键词:

生物可访问性生物可访问性常见的豆子常见的豆子类化合物类化合物蛋白质的水解蛋白质的水解.模拟的动态消化.粉的水解是粉的水解.

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