在熟练护理设施中检测碳烯耐药细菌 废水 废水
Susanna Lenz1, Subhasish Biswas2, Ivy Terry2,3
1Centers for Disease Control and Prevention, Division of Healthcare Quality Promotion (DHQP), Atlanta, Georgia, USA.
The Journal of infectious diseases
|November 18, 2025
概括
废水监测检测到在熟练护理设施中抗卡巴胺的细菌和卡巴胺酶基因. 这项研究展示了使用废水监测抗菌素耐药性威胁的概念验证.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物药物耐药性的监测监测
- 与医疗保健相关的感染
背景情况:
- 医疗保健设施的废水监测可以检测抗菌素耐药性 (AR) 威胁.
- 关于设施废水中的碳烯耐药 (CR) 细菌和碳烯酶基因的知识缺口存在.
研究的目的:
- 研究熟练护理设施 (SNF) 废水中的CR细菌和卡巴酶基因.
- 评估AR监测的文化依赖和文化独立的方法.
主要方法:
- 在六个月内从三个SNF收集了每周的废水样本.
- 使用选择性介质,PCR和全基因组测序 (WGS) 来识别CR细菌和AR基因.
- 对检测卡巴酶基因 (blaKPC,blaNDM,blaVIM,blaOXA-48-like,blaIMP) 的方法灵敏度进行比较.
主要成果:
- 确定了209个来自20个属的细菌分离物,包括临床相关的CR细菌.
- 在A和C设施中检测到blaKPC;在B设施中没有检测到碳烯酶基因.
- WGS证实了PCR结果与100%的一致性.
结论:
- 医疗机构的废水含有各种CR细菌和AR基因.
- 培养,PCR和测序方法为废水中的AR提供了洞察力.
- 废水监测为监测AR威胁提供了一个概念验证.
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