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实时同时监测细菌培养中的多种分析物

Maggie M Fink¹, Zahra Aljuboori¹, Maggie K Klaers²

¹Department of Chemistry and Biochemistry, Indiana University South Bend, South Bend, Indiana, USA.

Applied and environmental microbiology

|November 18, 2025

概括

研究人员开发了一种新的生物传感器,用于实时监测多种细菌代谢物. 这种工程化周等离子体结合蛋白 (PBP) 系统提供了一种非侵入性工具,用于跟踪细菌培养中的必需分子.

科学领域:

微生物学和生物技术
生物传感器开发开发
代谢工程是代谢工程.

背景情况:

细菌代谢物对健康,生态系统和工业至关重要.
目前的代谢物监测方法往往是离线的,劳动密集的,容易受到污染.
实时监测对于了解微生物动态和短暂代谢状态至关重要.

研究的目的:

开发一种用于同时实时监测多种细菌代谢物的新方法.
为细菌代谢物分析创建一种非侵入性,模块化和可扩展的工具.
为了证明工程周等离子体结合蛋白 (PBPs) 作为生物传感器用于此目的的应用.

主要方法:

使用与光体结合的工程周等离子体结合蛋白 (PBPs) 进行了利用.
该系统在细菌培养中进行了测试,包括*Escherichia coli*,*Pseudomonas aeruginosa*,*Staphylococcus aureus*,*Enterococcus faecalis*和*Clostridium striatum*.这些细菌培养中进行了测试.
在24小时内同时对葡萄糖,阿拉比诺斯, рибо斯,谷氨酸和氨酸等代谢物进行监测.

主要成果:

基于PBP的系统实现了多种代谢物的同时实时监测.
在24小时内保持了高时间分辨率和传感器稳定性.
在大肠杆菌和其他细菌物种中证实了层次化的基质利用.

结论:

基于PBP的多传感器阵列为细菌代谢物分析提供了多功能工具.
这种新的方法提供了非侵入性,模块化和可扩展的监控功能.
该技术有可能在基础微生物学和工业应用领域取得进展.

关键词:

细菌的新陈代谢是细菌的代谢.细菌周等离子体结合蛋白质生物传感器生物传感器光生物传感器生物传感器多传感器阵列阵列是一个多传感器阵列.

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