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Updated: Jan 11, 2026

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一个基于转录因子的框架,用于监测的压力状态:在保护和恢复中的应用
Shen-Tong Wang1,2,3, Li Li4,5,6,7,8, Qi Yang2
1Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, 266237, China.
Marine biotechnology (New York, N.Y.)
|November 18, 2025
概括
识别像Cg-CTCF和Cg-MYBA这样的转录因子 (TF) 可以有效地监测沿海的压力. 该框架结合了ATAC/RNA-seq和现场验证,用于海洋物种管理中的实际分子指标.
科学领域:
- 海洋生物学 海洋生物学
- 分子生态学分子生态学
- 环境压力生理学环境压力生理学
背景情况:
- 气候变化需要为沿海物种的压力提供分子指标.
- 单基因压力标志物受到复杂的多基因调控网络的限制.
- 转录因子 (TF) 提供了一种全面的方法来评估应激反应.
研究的目的:
- 制定一个框架,用于识别太平洋 (Crassostrea gigas) 中的TF压力指标.
- 验证已识别的TFs作为健康和管理的可靠分子标记.
主要方法:
- 将暴露在不同的潮条件下,以模拟压力.
- 对转化酶可访问的染色体测序 (ATAC-seq) 和RNA测序 (RNA-seq) 的测定,以确定关键的TFs.
- 在体内RNA干扰 (RNAi) 和现场验证以确认TF功能和适用性.
主要成果:
- 确定了CCCTC结合因子 (Cg-CTCF) 和MYB原瘤基因A (Cg-MYBA) 作为关键的TF.
- 这些TF在潮和热应激下得到了显著的升级.
- RNAi实验证实了它们在增强在压力下生存的作用.
- 现场验证显示,在自然压力条件下,这些基因的上调显著.
结论:
- Cg-CTCF和Cg-MYBA是太平洋压力的有希望的分子指标.
- 综合框架 (ATAC/RNA-seq,RNAi,现场验证) 在识别TF压力指标方面是有效的.
- 这种方法可以广泛应用于海洋生物的保护和管理策略.
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