在碳化合物污染的土壤中,微生物诱导石沉的可行性
Carla Comadran-Casas1, Philip J Salter2, Ayo Ogundero1
1James Watt School of Engineering, The University of Glasgow, UK. Carla.ComadranCasas@glasgow.ac.uk.
Environmental science. Processes & impacts
|November 18, 2025
概括
使用*Sproposarcina pasteurii*进行生物增量的微生物诱导石沉 (MICP) 有效地修复了用多环芳 (PAH) 处理的土壤. 这种方法克服了碳化合物抑制尿素水解的作用,这对于污染土壤中的重金属整治至关重要.
科学领域:
- 环境微生物学 环境微生物学
- 生物修复是一种生物修复.
- 土壤科学 土壤科学
背景情况:
- 微生物诱导的矿沉 (MICP) 是一种有前途的重金属生物修复技术.
- 有机污染物,如多环芳 (PAHs),经常与重金属共存,并可以通过影响尿素水解来抑制MICP.
- 在受PAH污染的土壤中,MICP的可行性尚不清楚.
研究的目的:
- 研究PAH污染土壤中MICP生物刺激和生物增强的潜力.
- 评估PAHs对细胞和酶水平的尿解的影响.
- 为了确定碳化合物对尿酶活性的抑制机制.
主要方法:
- 用土壤水提取物和土壤矩阵来测试MICP可行性.
- 使用流式细胞计量来评估细菌生长和细胞膜稳定性.
- 使用*Sprosaarcina pasteurii*和大豆尿酶在不同碳化合物度的存在下进行了尿素活性测试.
主要成果:
- 没有观察到本地尿溶性细菌的生物刺激.
- *S. pasteurii* 在土壤水提取物中生长,碳化合物度增加,而不会损坏膜.
- 碳化合物抑制尿酶活性是度依赖和竞争性的,影响细菌和植物尿酶,而细菌尿酶更敏感.
结论:
- 使用*S. pasteurii*进行生物增量,在受PAH污染的土壤中,在24小时内成功实现了尿素水解.
- 这项研究表明,S. pasteurii能够在受PAH污染的土壤中生长并保持尿解活性.
- 使用*S. pasteurii*的MICP是一种可行的策略,用于修复与重金属和PAHs共同污染的土壤.
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