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识别和长非编码RNA的表达分析,以应对VHSV感染的彩虹鱼

Mahmood Tavakoli-Alamouti¹, Ghodrat Rahimi-Mianji¹, Ardeshir Nejati-Javaremi²

¹Laboratory for Molecular Genetics and Animal Biotechnology, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Comparative biochemistry and physiology. Part D, Genomics & proteomics

|November 18, 2025

概括

受病毒性出血性败血症 (VHS) 感染的彩虹鱼表现出改变的长非编码RNA (lncRNA) 档案. 这些lncRNA及其相互作用是鱼类对病毒感染的免疫反应的关键.

科学领域:

水生动物的健康状况.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

病毒性出血性败血症 (VHS) 对水产养殖构成重大威胁.
长非编码RNAs (lncRNAs) 越来越多地被认为是它们在调节免疫反应中的作用.
关于彩虹鱼对VHS的ncRNA反应存在知识差距.

研究的目的:

在VHS病毒挑战之后,研究彩虹鱼脏中的非编码RNA (ncRNA) 档案.
确定ncRNAs在抗病毒免疫防御机制中的潜在作用.
分析lncRNA-mRNA相互作用和相关的信号通路.

主要方法:

来自VHS病毒挑战和控制彩虹鱼的脏的转录基因分析.
长非编码RNAs (lncRNAs) 的识别和分类.
对lncRNAs和信使RNAs (mRNAs) 的差异表达分析.
在差异表达的 lncRNA 和 mRNA 之间识别 cis 和 trans 相互作用.
路径丰富分析和定量特征位点 (QTL) 协会.

主要成果:

确定了4207个lncRNAs,包括新型异型和跨基因lncRNAs.
在病毒感染时检测到182个lncRNA和1326个mRNA的差异表达.
发现了特定的 cis 和 trans lncRNA-mRNA 相互作用 (例如,IL10-MSTRG.9783).
突出了PPAR,Toll-like受体和p53信号通路的参与.
与病毒应激相关的定量特征位置 (QTLs) 相关的 lncRNAs.

结论:

病毒感染显著改变彩虹鱼脏中的lncRNA表达.
lncRNAs在调节宿主对VHS的免疫反应中发挥着至关重要的作用.
已识别的lncRNA-mRNA相互作用和途径为抗病毒防御提供了分子洞察力.
QTL关联表明了 lncRNA介导的病毒耐药性的遗传基础.

关键词:

不同表达式的差异表达式高通量测序的高通量测序在 Oncorhynchus mykiss 的研究中.这是一个VHSVVV的视频.在 lncRNAs 中.

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