在无细胞生物转化中使用两种酶级联从1,3-二氨基中合成β-氨酸的一合成
Shanthi Shanmugasundaram1, Anitha Janet Roshni Yesudhas1, Andrea Kagoo1
1Centre for Biotechnology, Anna University, Chennai 600025, India.
Journal of bioscience and bioengineering
|November 18, 2025
概括
一种新型的酶过程使用两个酶从1,3-氨中合成β-氨酸. 这种一子方法,增强了料批生物转化,显著提高了β-氨酸生产效率.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 合成生物学 合成生物学
- 生物化学工程 生物化学工程
背景情况:
- 开发高效的生物催化路径,用于像β-氨酸这样有价值的化学物质至关重要.
- 酶合成为传统化学方法提供了一个可持续的替代方案.
- 单多酶级联在酶兼容性和辅因子再生方面存在挑战.
研究的目的:
- 从1,3-二氨 (DAP) 建立一种新的两种酶级联,用于从1,3-二氨 (DAP) 合成β-氨酸.
- 为了识别和表征具有改善该生物转化活性和稳定性的酶.
- 为了优化生物转化过程,使用食批量策略来克服基质抑制等局限性.
主要方法:
- 设计了一种涉及二胺氧化酶 (DAO) 和3-aminopropionaldehyde脱酶 (APALDH) 的两种酶级联.
- 使用了商业和重组酶,包括DAO,APALDH,catalase和NADH氧化酶.
- 确定了酶动力学和最佳反应条件 (pH,温度).
- 采用和比较了批量和养批量酶性生物转化策略.
主要成果:
- 一个概念证明合成使用真核DAO产生12.2mMβ-氨酸.
- 鉴定了来自Arthrobacter pascens (DAO_Ap) 的高活性DAO,在pH9.0和37°C时具有最佳活性.
- 用DAO_Ap进行批量生物转化,由于基质抑制,导致转化率低 (6%).
- 料批生物转化克服了基质抑制,实现了47%的转化并产生2.34毫米的β-氨酸.
结论:
- 从DAP成功开发了一种新且高效的两种酶级联,用于从DAP中合成单β-氨酸.
- 料批次酶生物转化通过克服基质抑制,显著提高了β-氨酸产量.
- 这项研究表明,对于β-氨酸的可持续生产,有前途的生物催化策略.
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