相关实验视频
Updated: Jul 25, 2026
08:56
Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats
Published on: April 7, 2023
1.2K
可以减轻德甲诱导的骨质母细胞功能障碍,并通过PI3K/AKT/GSK3β通路激活途径预防大腿骨
Sun Xuecheng1, Chen Changjun2, Ma Xiaojie3
1Clinical School/College of Orthopedics, Tianjin Medical University, Tianjin, China.
Orthopaedic surgery
|November 18, 2025
概括
补充剂通过激活PI3K/AKT/GSK3β通路,可以防止葡萄糖皮质醇诱导的股骨头骨硬化 (GC-ONFH) 的骨损失. 这种方法为风险患者的GC-ONFH治疗提供了一个有希望的策略.
科学领域:
- 生物医学科学 生物医学科学
- 骨生物学 骨生物学 骨生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 骨骨头的葡萄糖皮质激素诱导骨 necrosis (GC-ONFH) 是类固醇治疗的严重并发症,其特征是骨质细胞功能障碍和骨形成受损.
- (Se) 以其骨保护作用而闻名,但其在GC-ONFH中的特定作用和机制尚不清楚.
研究的目的:
- 在GC-ONFH的老鼠模型中研究口服补充剂的治疗疗效.
- 阐明减轻GC-ONFH的潜在分子机制.
主要方法:
- 使用甲基prednisolone糖酸盐 (MPS) 建立了GC-ONFH的老鼠模型.
- 鼠被用治疗了4周. 在体外研究中使用了用德甲 (DEX) 治疗的原发性骨质母细胞和MC3T3-E1细胞.
- 评估包括微型CT,组织形态测量,细胞增殖试验,流细胞测量,RT-qPCR,西部斑点和免疫光.
主要成果:
- 补充剂在GC-ONFH大鼠中预防了椎体缩,并减少了空隙.
- 逆转了DEX诱导的损伤,恢复了细胞增殖,抑制了细胞亡,并增强了骨质生成活性.
- 在机械上,激活PI3K/AKT/GSK3β信号通路,促进骨质细胞功能.
结论:
- 补充剂通过激活PI3K/AKT/GSK3β通路,有效地减弱了大鼠模型中的GC-ONFH.
- 可以逆转DEX诱导的细胞功能障碍,并恢复骨质生成能力.
- 补充剂是管理GC-ONFH的临床适用策略.
相关概念视频
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...