膀过活的儿童尿道微生物组合的变化:来自16S rRNA基因测序的见解
Jiajie Li1, Longdi Yu1, Yu Wang2
1Department of Pediatric Surgery, West China Hospital, Sichuan University, No.37 Guoxue Lane, Wuhou District, Chengdu, 610041, Sichuan, PR China.
Scientific reports
|November 18, 2025
概括
儿科过度活性膀 (OAB) 与尿路微生物组的破坏有关,在OAB患者中发现了像Escherichia-Shigella这样的特定细菌. 这种失生症可能在OAB的发展和严重性中发挥作用.
科学领域:
- 微生物学 微生物学
- 儿科泌尿外科 儿科泌尿外科
- 泌尿生殖系统医学
背景情况:
- 过度活性膀 (OAB) 是儿童常见的疾病,影响生活质量.
- 尿道微生物组在儿科OAB病原体中的作用仍然在很大程度上未被探索.
- 了解微生物社区结构对于开发向疗法至关重要.
研究的目的:
- 描述小儿OAB患者与健康对照者的尿道微生物群概况.
- 为了确定在OAB中差异丰富的特定微生物种群.
- 探索尿路微生物群和OAB临床症状之间的关联.
主要方法:
- 分析了87名5至14岁儿童 (39名OAB,48名对照) 的尿样.
- 使用Illumina NovaSeq6000进行了16S rRNA基因测序 (V3-V4区域).
- 生物信息学和统计分析 (PERMANOVA,PCoA,LEfSe,Spearman相关性) 用于评估多样性,种类和临床相关性.
主要成果:
- 在OAB患者和对照人群之间观察到明显的尿道微生物群落 (β-多样性分析).
- 在OAB患者中,蛋白质细菌,蛋白质细菌,肠杆菌,斯芬戈蒙纳斯和埃舍里希亚-希格拉菌的丰富性被发现.
- 对照组的Clostridia,Bacteroidales和Prevotella含量较高,功能预测表明OAB相关的抗生素耐药性和生物膜形成.
结论:
- 儿科OAB与具有促炎性细菌和改变代谢功能的异生性尿路微生物群有关.
- 特定的微生物特征,如Escherichia-Shigella可能有助于OAB的发病.
- 这些发现表明,在儿科OAB中,有潜在的微生物组信息的诊断和治疗策略.
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