相关实验视频

Updated: Jun 24, 2026

A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method

A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method

Published on: September 30, 2017

一种超声波导向的活检技术,用于获得关节上棕色脂肪活检和前脂肪细胞

Eline Sandvig Andersen¹, Naja Zenius Jespersen¹, Tora Ida Henriksen¹

¹Centre for Physical Activity Research, Copenhagen University Hospital - Rigshospitalet, Rigshospitalet, section 7641 Blegdamsvej 9, Copenhagen, DK-2100, Denmark.

Scientific reports

|November 18, 2025

概括

研究人员开发了一种超声波引导的活检方法来研究人类棕色脂肪组织 (BAT). 虽然在获取样本方面取得了成功,但为了在未来的代谢疾病研究中获得更高的成功率,需要优化程序.

科学领域:

代谢性疾病研究研究.
脂肪组织生物学脂肪组织生物学
医疗成像应用医疗成像应用

背景情况:

棕色脂肪组织 (BAT) 从葡萄糖和脂肪酸中产生热量,为肥胖和代谢障碍提供治疗点.
人类BAT的体内研究是具有挑战性的,因为它的解剖位置,特别是在关节上部.

研究的目的:

建立超声波导向活检技术,以获取小型人类关节上脂肪组织样本.
从这些活检中开发RNA提取和脂肪原生细胞分离的程序.
评估研究人类BAT特征和原始细胞分化潜力的可行性.

主要方法:

超声波导向超关节区域活检用于小组织采集.
定量PCR (qPCR) 分析的标准化RNA提取协议.
脂肪原生细胞的隔离和培养,以评估分化能力.

主要成果:

从小型活检中提取了足够的RNA用于qPCR分析.
脂肪原始细胞被成功分离出来,并显示出一些分化能力.
总体成功率很低,样品和培养中的差异化有限,BAT概况不一致.

结论:

该研究为人类BAT研究的超声波导向活检方法提供了概念验证.
讨论了方法的改进,以提高未来临床干预研究的成功率.
该技术为深入研究人类BAT生物学及其治疗应用提供了潜在的途径.

更多相关视频

Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique

Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique

Published on: September 27, 2021

Author Spotlight: Optimizing Supraclavicular Brown Adipose Tissue Extraction for Genetic Analysis

Author Spotlight: Optimizing Supraclavicular Brown Adipose Tissue Extraction for Genetic Analysis

Published on: March 29, 2024

相关概念视频

Ultrasonography

Ultrasonography

Ultrasonography is an imaging technique that uses high-frequency sound waves to visualize the body's internal structures. It is a non-invasive and safe procedure that does not involve the use of ionizing radiation, making it widely used in various medical fields. Ultrasonography is used to study heart function, blood flow in the neck or extremities, certain conditions such as gallbladder disease, and fetal growth and development.
During an ultrasonography procedure, a handheld device called a...

Ultrasound II: Endoscopic Ultrasound and FibroScan

Ultrasound II: Endoscopic Ultrasound and FibroScan

Endoscopic Ultrasound (EUS) and FibroScan are valuable diagnostic tools in gastroenterology and hepatology, each with specific applications and techniques.
Endoscopic Ultrasound (EUS):