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相关概念视频

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

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卷度吸收微样采集用于光度分析:方法验证和稳定性评估.

Elisa Milandri1, Roberta Di Lecce2, Chiara Pia Iattoni1

  • 1Research Group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy.

Analytical and bioanalytical chemistry
|November 18, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的微量采样方法,用于分析抗精神病药物卢马特佩龙. 这种方法支持治疗药物监测 (TDM) 以实现个性化精神病治疗,改善患者的坚持和减少副作用.

关键词:
在HPLCMS/MS/MS中使用.一个是Lumateperone.方法开发和验证方法的发展和验证.微量采样是指微量采样.固体相提取 (SPE) 是一种卷度吸收微采样 (VAMS) 是一种微采样技术.

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科学领域:

  • 药理学 药理学是指药理学的学科.
  • 分析化学 分析化学
  • 临床诊断 临床诊断 临床诊断

背景情况:

  • 卢马特是治疗精神分裂症的非典型抗精神病药,具有中度副作用.
  • 治疗药物监测 (TDM) 对于个性化治疗和降低毒性风险至关重要,特别是在多种药物同时使用的情况下.
  • 微采样为TDM提供了优势,包括最小侵入性采集,增强分析剂稳定性和简化后勤.

研究的目的:

  • 开发和验证基于微样本的分析方法,以量化lumateperone.
  • 为了比较体积吸收微采样 (VAMS) 与传统的血分析对lumateperone的性能.
  • 为了支持未来TDM应用程序的精神病患者用lumateperone治疗.

主要方法:

  • 开发和验证一种高性能液体染色学-双重质谱法 (HPLC-MS/MS) 方法.
  • 使用体积吸收微采样 (VAMS) 进行毛细血管血液采集.
  • 将VAMS结果与标准的管内流体血分析和进行的稳定性测试进行比较.

主要成果:

  • 一个可靠和精简的VAMS协议被优化为lumateperone分析.
  • 该HPLC-MS/MS方法证明了令人满意的线性,精度,提取产量和矩阵效应.
  • VAMS的性能与血分析相当,在干燥的微样本中观察到增强的分析剂稳定性.

结论:

  • 这是第一个经过验证的微采样方法用于光烯分析,提供可靠的数据,可与血分析相提并论.
  • 开发的VAMS方法在采集,储存和处理方面比传统的血液采样具有显著的优势.
  • 这种方法对未来的TDM应用在接受甲治疗的患者中显示出希望.