在食品衍生菌株Levilactobacillus brevis M3R3中的胆汁盐水解酶活性:基因组和功能性特征
Gianluigi Agolino1, Marianna Cristofolini2, Maria Anna Ronsivalle2
1Department of Agricultural, Food and Environment, University of Catania, 100, 95123, Catania, Italy.
Probiotics and antimicrobial proteins
|November 19, 2025
概括
一种酸面细菌,Levilactobacillus brevis M3R3,表现出胆汁盐酸酶 (BSH) 活性,并分解胆汁盐. 基因组分析证实了其对功能性食品的安全性和益生菌潜力.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- 胆盐化酶 (BSH) 的活性对于益生菌在胃肠道中生存至关重要.
- 列维拉克托巴西勒斯 (Levilactobacillus brevis) 菌株以其潜在的益生菌特性而闻名.
- 了解BSH活动和安全性是开发功能性食品成分的关键.
研究的目的:
- 为了描述西西里酸面中的Levilactobacillus brevis M3R3的胆盐酸酶 (BSH) 活性.
- 通过基因组和功能分析来评估L. brevis M3R3的安全性和益生菌潜力.
- 在模拟的肠道条件下研究菌株对胆汁盐的脱.
主要方法:
- 使用UHPLC/HR-MS.的动态生长试验和胆盐脱分析.
- 全基因组测序和in silico安全性评估.
- 在不同的条件下对已识别的bsh基因进行基因表达分析.
主要成果:
- L. brevis M3R3表现出对单个胆盐的耐受性,并适应混合胆盐溶液.
- 这种菌株完全解了葡萄糖结合胆汁盐,部分解了牛结合胆汁盐.
- 基因组测序显示没有抗微生物耐药性或毒性基因,并确定了两个表达的bsh基因 (bsh_2A和bsh_3A).
结论:
- L. brevis M3R3是一种安全的菌株,具有显著的胆盐化酶 (BSH) 活性.
- 这种菌株具有功能性bsh基因,在胆盐暴露下表达.
- L. brevis M3R3显示出作为促进健康配方中的功能成分的潜力.
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