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Updated: Jan 11, 2026

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改善结核病诊断:评估舌头抽样作为一种补充分析的方法在几内亚
Souleymane Hassane-Harouna1, Moussa Condé2, Kindy Bah2
1Damien Foundation, Conakry, Guinea. hassoul20@yahoo.fr.
Annals of clinical microbiology and antimicrobials
|November 19, 2025
概括
舌头扫描 (TS) 显示了使用分子测试诊断肺结核 (PTB) 的前景,为提供了一个不那么侵入性的替代品. 对于无法产生唾液的患者,需要进一步的研究.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断学
背景情况:
- 肺结核 (PTB) 诊断通常依赖于唾液检查,对于无法产生唾液的患者来说,这是困难的.
- 作为PTB诊断的替代方法,正在探索微创性口腔采样方法,如舌头拭子 (TS),作为PTB诊断的替代方法.
- 评估TS的有效性与用于检测PTB的分子测试结合,至关重要.
研究的目的:
- 通过分子诊断试验,评估舌头扫描 (TS) 用于诊断肺结核 (PTB) 的有效性.
- 为了比较TS的诊断性能与涂抹阳性PTB患者的传统唾液样本 (SS).
- 在对结核病症状患者的回顾性分析中调查TS的实用性.
主要方法:
- 一项前性研究涉及99名涂抹阳性PTB患者,为GeneXpert-Ultra测试提供TS和唾液样本 (SS).
- 单独 (1TS) 和组合 (3TS) 的TS与SS一起进行了测试,以检测Mycobacterium结核病 (MTB).
- 一个回顾性分析使用内部的IS6110-qPCR对120名有症状的个体的TS进行了回顾性分析,比较了基于唾液的显微镜,GeneXpert-MTB/RIF (Xpert) 和Ultra的结果.
主要成果:
- 在前性队列中,GeneXpert-Ultra检测到1TS的86.9%和3TS的91.9%的MTB,而样中的96.9%.
- 虽然唾液样本显示出更高的检测率和阳性等级,但TS显示出MTB的分子检测,阳性等级大多为"MTB低".
- 追溯分析显示,基于唾液的分子测试 (Xpert,Ultra) 和TS的内部IS6110-qPCR (分别为0.91和0.86的kappa值) 之间存在强烈一致.
结论:
- 舌头拭子 (TS) 可以通过分子测试有效检测出Mycobacterium结核病 (MTB).
- TS代表了肺结核 (PTB) 诊断的最小侵入性补充工具.
- 需要进一步的研究,特别是在有限的唾液产量患者群体中.
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