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与胚胎发育和猪胚胎DNA损伤反应相关的miRNAs的表达

Gabriel Pineda¹, Fernanda Luiza Facioli¹, Mariana Priotto de Macedo¹

¹Department of Animal Science, McGill University, Sainte Anne de Bellevue, Quebec, Canada.

Molecular reproduction and development

|November 19, 2025

概括

这项研究探讨了猪胚胎中的微RNA (miRNA) 在基因组激活和DNA损伤反应过程中. 我们确定了在发育过程中和DNA损伤后发生变化的特定miRNA,为胚胎健康提供了新的见解.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发育生物学是发展生物学.

背景情况:

胚胎基因组激活 (EGA) 和DNA损伤反应 (DDR) 对胚胎发育至关重要.
表观遗传机制调节了EGA和DDR.
微RNAs (miRNAs) 在猪胚胎EGA和DDR中的作用未被描述.

研究的目的:

在EGA期间在猪胚胎中表达miRNA的特征.
在诱导DNA损伤后调查miRNA变化.
在猪胚胎中探索miRNAs,EGA和DDR之间的联系.

主要方法:

在四个发育阶段 (D2,D3,D4,胚胎囊) 评估了miRNA的丰度.
使用紫外线暴露诱导的DNA损伤,并通过γH2AX免疫光验证.
量化了EGA相关基因的mRNA水平,以确认发育时间点.

主要成果:

在猪胚胎发育过程中确定了特定miRNAs (miR-200a-5p,miR-15a,miR-24-3p) 的动态变化.
紫外线诱导的DNA损伤导致胚胎细胞率和细胞数量下降.
DNA 损伤改变了胚胎囊中的特定miRNAs (miR-29a-3p,miR-344b-3p) 的表达,并在D4.4时影响了miR-15a和miR-24-3p水平.

结论:

这项研究首次描述了猪胚胎在EGA期间miRNA表达的特征.
特定的miRNA参与了猪胚胎对DNA损伤的反应.
这些发现突出了miRNAs在调节猪胚胎发育和DNA完整性方面的潜在作用.

关键词:

DNA 双链断裂发生紫外线辐射紫外线辐射胚胎发育过程中的胚胎发育.胚胎基因组激活活动这是一个小RNARNA.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...