细胞保留技术在Komagataella phaffii实验室级连续过程中的作用
Marina Y Linova1, Satish K Kodiripaka1, Edite Martins1
1Department of Chemical and Biochemical Engineering, Technical University of Denmark, Kongens Lyngby, Denmark.
Biotechnology progress
|November 19, 2025
概括
这项研究比较了Komagataella phaffii连续生物制造的声学和基于膜的 perfusion 过程. 基于声学的过程显示出更高的生产率和细胞保留效率,用于抗体片段的生产.
科学领域:
- 生物技术是生物技术.
- 生物工艺工程 生物工艺工程
- 细胞培养技术 细胞培养技术
背景情况:
- perfusion 技术对于持续的生物治疗生产至关重要,主要是在哺乳动物系统中.
- perfusion 对其他宿主如 Komagataella phaffii 的应用是有限的.
- 有效的细胞保留对于 perfusion 生物反应器的性能至关重要.
研究的目的:
- 研究和比较两个实验室规模的细胞保留技术,用于Komagataella phaffii连续 perfusion 过程.
- 评估基于声学 (AP) 和基于膜 (MP) 的工艺在生产率,可活性和细胞密度方面的性能.
- 评估这些技术是否适用于基于酵母的连续生物制造.
主要方法:
- 使用声学细胞分离器 (ACS) 开发基于声学的工艺.
- 开发一种基于膜的工艺,使用振动膜过装置 (VMF).
- 持续培养Komagataella phaffii用于scFv13R4抗体片段的产生,持续8-9天.
主要成果:
- 在长时间内,AP和MP都保持了高细胞活力 (>90%) 和高生产率.
- 与MP (32.29 mg L−1 day−1 和 0.44 mg g−1 day−1) 相比,AP实现了较高的体积 (50.63 mg L−1 day−1) 和特异性 (1.09 mg g−1 day−1) 的生产率.
- MP达到100%的分离效率,生物质高达74.1g L-1 DCW,而AP以55.1g L-1 DCW达到98%的效率,显示出对较大的酵母细胞的选择性.
结论:
- 基于声学的细胞保留在Komagataella phaffii perfusion过程中提供了更高的生产率.
- 基于膜的过实现了更高的生物质度,但具有较低的特定生产率.
- 这两种技术都适用于K. phaffii连续 perfusion,声学分离显示了提高产量的潜力.
关键词:
科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.声音细胞分离的声音细胞分离.细胞保留 细胞保留持续的生物处理.perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion 这种方法通常被认为是最常见的方法之一,因为它可以让一个人的身体变得更加舒适,可以让一个人的身体变得更加舒适.蛋白质生产 蛋白质生产振动膜过器的过方式
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