通过集成的非病毒基因传递,近视频光遗传学和电生理记录来进行深度大脑调制的闭环神经接口平台
Chao-Yi Chu1, Zih-Huei Chen2, Chun-Wei Liang1
1Department of Materials Science and Engineering, National Yang Ming Chiao Tung University, No. 1001, Daxue Rd., Hsinchu, 300093, Taiwan, ROC.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 19, 2025
概括
这项研究引入了一种新的,无纤维的神经接口,用于精确的,细胞特异的神经调节. 该设备可实现非病毒基因传递和远程光遗传刺激,增强深部大脑电路控制.
科学领域:
- 神经科学是一个神经科学.
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
背景情况:
- 传统的光遗传学面临着侵入性,病毒基因传递和硬件复杂性的挑战.
- 精确,稳定和细胞特异的神经电路控制对于闭环神经调节至关重要.
研究的目的:
- 开发一种多功能,可植入的神经接口,用于无纤维,非病毒光遗传控制.
- 将基因传递,光遗传刺激和电生理学记录集成到一个单一的设备中.
- 为了证明深层大脑神经调节的体内疗效.
主要方法:
- 用于基因转移和信号记录的3D黄金逆光 (AuIO) 微电极的制造.
- 使用与NT-PEI复合的Channelrhodopsin-2 (ChR2) 质粒进行非病毒基因传递.
- 通过气溶喷射打印将近红外 (NIR) 光转换为蓝光的上转换纳米粒子 (UCNPs) 的集成.
- 在体内植入海马牙状回 (DG) 进行功能评估.
主要成果:
- 在神经元中成功的非病毒传递和ChR2的表达.
- 无纤维,使用NIR光实现通过LSPR增强的远程光遗传刺激.
- 实时,光唤起的神经活动记录在体内.
- 在体内表现出稳定的 in vivo 操作和生物相容性.
结论:
- 开发的全合一平台为深层大脑光遗传工程提供无纤维,生物相容的解决方案.
- 这项技术通过简化外科手术要求和增强控制来推进精确的闭环神经调节.
- 多功能接口对未来针对神经回路的治疗应用具有前景.
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