在腹部手术后,针对肠道屏障功能障碍的血清克劳丁-3和佐努林的诊断研究
YiChen Hu1, WenShuo Liu2, XueJing Gong3
1Department of Gastrointestinal Surgery, Second Affiliated Hospital of Kunming Medical University, Kunming, China.
在手术后肠道屏障功能障碍时,血清中的克劳丁-3和佐努林水平会增加. 这些生物标志物对手术后肠道屏障问题具有良好的预测价值,其中克劳丁-3更有效.
科学领域:
- 胃肠病学 胃肠病学
- 手术研究的研究.
- 生物标志物发现发现
背景情况:
- 肠道屏障功能至关重要,可以使用血清克劳丁-3和佐努林来评估.
- 手术后肠壁功能障碍是腹部手术后的一个重大临床问题.
研究的目的:
- 评估血清克劳丁-3和佐努林在腹部手术后检测肠道屏障功能障碍时的诊断效用.
- 在这种情况下,要比较血清克劳丁-3与佐努林的诊断准确性.
主要方法:
- 建立了肠道缺血-再输液 (I/R) 损伤的老鼠模型,通过HE染色来评估粘膜损伤.
- 与肠道粘膜损伤的严重程度相关的血清Claudin-3和Zonulin水平.
- 在接受腹部手术的患者中测量了血清中的克劳丁-3,佐努林和周围血液Escherichia coli 16sDNA.
- 使用的接收器操作特征 (ROC) 曲线来确定诊断值.
主要成果:
- 在大鼠中,血清中的Claudin-3和Zonulin水平反映了肠道粘膜损伤,在再输血后24小时达到峰值,并显示出显著的正相关性.
- 在手术患者中,在手术后24小时内观察到血清中Claudin-3,Zonulin和E. coli 16sDNA的升高,这些患者有障碍功能障碍.
- 在ROC曲线分析中,克劳丁-3的曲线下面面积 (AUC) 为0.934和佐努林的0.826.
结论:
- 血清中克劳丁-3和佐努林度与肠粘膜损伤程度和术后肠壁功能障碍正相关.
- 血清中的Claudin-3和Zonulin作为术后肠道屏障功能障碍的有价值的预测生物标志物.
- 血清克劳丁-3在确定术后肠道屏障功能障碍时,与血清佐努林相比,具有更高的诊断准确性.
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