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Updated: Jan 11, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

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人类线粒体DNA的向删除是通过V型CRISPR-Cas12a系统进行的

Natalia Nikitchina1, Anne-Marie Heckel1, Nikita Shebanov1

  • 1UMR7156-Molecular Genetics, Genomics, Microbiology, CNRS/University of Strasbourg, Strasbourg 67000, France.

NAR molecular medicine
|November 19, 2025
PubMed
概括

研究人员开发了一个基于CRISPR的系统,精确编辑线粒体DNA (mtDNA). 这种工具可以创建特定的mtDNA删除,有助于研究和建模线粒体疾病.

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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