Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Walras modulates sex-dependent endoplasmic reticulum stress in cardiomyopathy.

Frontiers in physiology·2026
Same author

Chd4 and ThPOK cooperate to preserve structural and electrophysiological integrity of the adult heart through Sprr1a repression.

The FEBS journal·2026
Same author

Cancer-associated fibroblasts regulate DNA repair in pancreatic cancer through NDRG1-mediated R-loop processing.

Nature cell biology·2026
Same author

Pinhole engineering based enhanced resolution (PEER) for fluorescence lifetime imaging microscopy.

Communications biology·2026
Same author

PSF-Driven Spatio-Temporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.

bioRxiv : the preprint server for biology·2026
Same author

Plastic debris budget and fluxes along the Barcelona coastline.

The Science of the total environment·2026
Same journal

Somatosensory cortex shapes perceptual decision bias via the superior colliculus.

Research square·2026
Same journal

Combinatorial Targeting of Avapritinib-Driven MAP Kinase Activation in High-Grade Glioma.

Research square·2026
Same journal

Supporting Implementation of the National Standards for Cancer Survivorship Care: Development of the Cancer Survivorship Maturity Model (CSMM).

Research square·2026
Same journal

Operationalizing a walking exercise prescription based on 6-minute walk test results.

Research square·2026
Same journal

Age but not sex modifies lymphoid immune responses in murine sepsis.

Research square·2026
Same journal

Indirect effect, through aspects of neighborhood affluence and racial/ethnic composition, of receiving a Section 8 voucher on the prevalence of psychiatric disorders among boys and girls in the Moving to Opportunity study.

Research square·2026
查看所有相关文章

相关实验视频

Updated: Jan 11, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

25.9K

基于针孔工程的增强分辨率 (PEER) 光终身成像显微镜.

Wonsang Hwang1, Sinyoung Jeong2, J Matthew Dubach3

  • 1Wellman Center for Photomedicine, Harvard Medical School, Massachusetts General Hospital, CNY149, 13th St, Charlestown, 100190, MA, USA.

Research square
|November 19, 2025
PubMed
概括
此摘要是机器生成的。

我们使用针孔工程开发了一种新的差异共聚焦显微镜方法,以提高成像分辨率和深度. 这种技术为生物研究提供了更简单,高分辨率的光终身成像显微镜 (FLIM).

关键词:
与焦点相对的焦点相对的飞行员飞行员的飞行员子 一个子超级分辨率的超级分辨率

更多相关视频

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

1.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.2K

相关实验视频

Last Updated: Jan 11, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

25.9K
Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

1.1K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.2K

科学领域:

  • 显微镜的使用方法
  • 生物光子学 生物光子学
  • 超高分辨率成像成像技术

背景情况:

  • 混焦显微镜提供光学切割,但受到衍射的限制.
  • 在显微镜中提高横向分辨率和轴深度对于生物研究至关重要.
  • 光终身成像显微镜 (FLIM) 提供了定量分子信息,但通常需要复杂的设置.

研究的目的:

  • 为了增强差异共聚焦显微镜的侧面分辨率和轴切割深度.
  • 开发一种更简单,超高分辨率的光终身成像显微镜 (FLIM) 技术.
  • 为了实现高分辨率,多重复合的FLIM,用于先进的亚细胞成像.

主要方法:

  • 在差异共聚焦显微镜中实施了针孔工程.
  • 整合了一个强度加权的终身成像策略.
  • 通过对光校准幻灯片和亚细胞结构进行模拟和实验来验证该技术.

主要成果:

  • 在横向分辨率上实现了1.6倍的改进.
  • 证明轴切割能力增加了两倍.
  • 获得的量化生命周期数据与领先的超分辨率FLIM技术相比,实施更简单.

结论:

  • 新的差异共聚焦显微镜技术显著提高了成像性能.
  • 综合强度加权终身成像策略为超分辨率FLIM提供了一条可访问的途径.
  • 这种方法推进了用于生物应用的高分辨率,复合成像.