Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Thousandfold Expansion Microscopy.

bioRxiv : the preprint server for biology·2026

Same author

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026

Same author

Mutation E300 recommended by protein language models gives ChrimsonR amplified photocurrent response.

bioRxiv : the preprint server for biology·2026

Same author

Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ.

bioRxiv : the preprint server for biology·2026

Same author

A myoneural actuator with engineered biophysics for implantable biohybrid systems.

Nature communications·2026

Same author

Three-dimensional nanophotonics with spatially modulated optical properties.

Light, science & applications·2026

Search research articles

相关实验视频

Updated: May 5, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

通过政策优化设计具有减少T细胞表位的蛋白质.

Manvitha Ponnapati, Sapna Sinha, Brian Lynch

bioRxiv : the preprint server for biology

|November 19, 2025

概括

设计治疗性蛋白质需要评估免疫系统的兼容性. 这项研究开发了计算工具,通过建模蛋白质体分裂,化和主要基因相容性复合体 (MHC) 第I类结合来预测和最小化免疫反应,从而增强蛋白质设计以获得临床成功.

科学领域:

计算生物学是一种计算生物学.
免疫信息学是指免疫信息学.
蛋白质工程是一种蛋白质工程.

背景情况:

深度生成模型正在推进用于治疗的蛋白质设计.
临床成功取决于免疫系统的兼容性,包括蛋白质体分裂,化和主要基因相容性复合体 (MHC) 第I类结合.
以前的模型通常将这些免疫反应步骤单独分析或对有限的MHC等位基因进行分析.

研究的目的:

为MHC Class I路径相互作用开发集成的计算预测器.
将使用证据深度学习的不确定性估计纳入其中.
为了生成针对各种MHC等位基因的免疫兼容性优化的蛋白质候选者.

主要方法:

在人类蛋白质上微调蛋白质语言模型.
利用群体相对政策优化 (GRPO) 来减少MHC I类表现.
实施课程学习框架,逐渐增加复杂性 (掩盖表位和MHC等位基因).

主要成果:

开发统一的不确定性估计,用于切割,化和约束预测.
一个蛋白质语言模型的成功对齐,以最大限度地减少MHC I类表位.
展示一种产生免疫兼容蛋白质候选者的策略.

更多相关视频

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

Published on: August 16, 2019

相关实验视频

Last Updated: May 5, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

Published on: August 16, 2019

结论:

开发的预测剂提供了一种统一的方法,用于在MHC Class I途径中建模免疫反应.
GRPO和课程学习策略有效地减少了跨多种MHC等位基因的免疫原性表位.
这项工作通过考虑预测不确定性,推动了更安全,更有效的蛋白质疗法的设计.