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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

11.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.7K

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相关实验视频

Updated: Jan 11, 2026

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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通过DMS MaP-Seqq可视化的核糖体诱导的mRNA伪结相互作用.

Preston A Kellenberger1, Zhenwei Song1, Xiao Heng1

  • 1Department of Biochemistry, University of Missouri, Columbia, MO, USA.

bioRxiv : the preprint server for biology
|November 19, 2025
PubMed
概括

核糖体通过特定的键稳定RNA伪结,但也使它们不依赖于其位置占用而变得不稳定. 这项研究强调了DMS MaP-Seqq.

科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 病毒学 病毒学

背景情况:

  • RNA动态对于生物功能至关重要,在翻译过程中,核糖体与各种RNA结构相互作用.
  • 已知RNA伪结会阻碍核糖体读取框架的维护.
  • 了解伪结节-核糖体相互作用是破译翻译忠实性的关键.

研究的目的:

  • 为了研究由核糖体诱导的RNA伪结的构造变化.
  • 描述甘黄叶病毒伪结 (ScYLV_PK) 的稳定性和展开机制.
  • 建立二甲基硫酸盐探测与突变分析和测序 (DMS MaP-Seq) 作为研究核糖体-RNA相互作用的工具.

主要方法:

  • 利用二甲基硫酸盐探测与突变分析和测序 (DMS MaP-Seq) 来分析RNA结构.
  • 应用DMS MaP-Seq来检查糖黄叶病毒伪结 (ScYLV_PK) 在核糖体的存在下展开的情况.
  • 研究了特定残留物和核糖体占用率 (A/P位) 在伪结结稳定性中的作用.

主要成果:

  • ScYLV_PK的螺旋连接由核糖体稳定,取决于在残留27的结.
  • 核糖体破坏野生类型ScyLV_PK的稳定,独立于A/P位点占用.
关键词:
结构 RNA 结构 RNA 结构核糖体 核糖体 核糖体 核糖体它们是mRNARNA.

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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  • DMS MaP-Seq精确地检测到由核糖体诱导的RNA结构变化.

    • 结论:

    • DMS MaP-Seq是一种敏感的方法,用于检测核糖体介导的RNA结构动态.
    • 特定的结构图案,包括在残留物27的键,决定了转化过程中伪结的稳定性.
    • 核糖体与伪结的相互作用涉及复杂的稳定和不稳定机制.