有氧发酵的融合进化通过不同的机制,作用于关键的共享甘油性基因
Linda C Horianopoulos1,2, Antonis Rokas3, Chris Todd Hittinger1
1Laboratory of Genetics, J. F. Crow Institute for the Study of Evolution, Center for Genomic Science Innovation, DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, WI 53726, USA.
bioRxiv : the preprint server for biology
|November 19, 2025
概括
研究人员发现了一种酵母属,Saturnispora,它融合进化了有氧发酵. 与Saccharomyces cerevisiae相比,这种特征是通过不同的遗传机制产生的,突出了进化约束.
科学领域:
- 进化生物学 进化生物学
- 分子生物学分子生物学
- 酵母基因组学 酵母基因组学
背景情况:
- 了解进化趋同和约束是解释多样化的关键.
- 细菌菌通过整个基因组复制和糖性基因保留进化了有氧发酵.
研究的目的:
- 为了研究各种酵母物种的糖解率.
- 为了确定酵母中的有氧发酵的融合进化.
- 阐明融合有氧发酵背后的遗传机制.
主要方法:
- 开发并部署了一种细胞外酸化率 (ECAR) 试验.
- 分析了299种酵母菌种,跨越了4亿多年的进化.
- 利用了比较基因组学和转录基因组学.
- 在Saturnispora dispora进行基因删除实验.
主要成果:
- 鉴定了Saturnispora属中的一个分类,该分类融合进化了有氧发酵.
- 在有氧发酵Saturnispora的糖溶性基因中发现了更高的表达和新的cis-regulatory元素.
- 在Saturnispora dispora中删除一个关键的转录因子,降低了糖溶性速率和增加了呼吸.
- 在S. cerevisiae中发现了基因对象对重复的糖溶性基因的升高调节.
结论:
- 与S. cerevisiae相比,Saturnispora中的有氧发酵的融合进化涉及不同的遗传机制.
- 不同的遗传路径表明有氧发酵的出现存在强烈的进化约束.
- 对酵母进化的比较分析揭示了对代谢特征的保存和新型调节策略.
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