双Ca2+传感器在突触囊外细胞突变中的进化保守和分歧机制
bioRxiv : the preprint server for biology
|November 19, 2025
概括
这项研究揭示了两个传感器SNT-1和SNT-3是如何通过与SNARE复合体相互作用来控制C. elegans中神经递质释放的. 它们使用不同的快速和缓慢释放策略,SNT-1也调解自发释放.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 神经递质释放对于突触功能至关重要.
- 双 Ca2 + 传感器系统,如哺乳动物中的突触胺-1 和 -7,调节了这一过程.
- SNT-1和SNT-3是这些哺乳动物传感器的C. elegans同类.
研究的目的:
- 研究SNT-1和SNT-3调解神经递质快速和缓慢释放的机制.
- 探索它们与SNARE复合体的相互作用及其多基基图案的作用.
- 了解突触外细胞调节的进化保护和分歧.
主要方法:
- 使用AlphaFold 3来建模SNT-1/SNT-3-SNARE复合体.
- 进行了针对性突变发生的电生理学分析.
- 确定了保留的结合残留物和功能域.
主要成果:
- 阿尔法模型证实了保存的Syt1 C2B-SNARE相互作用.
- 无论是SNT-1还是SNT-3,都需要C2B-SNARE相互作用和多基动机来引起释放.
- 对SNARE接口和多基因模式的差异依赖表明了不同的释放策略.
- SNT-1通过多个途径调解自发释放.
结论:
- 在SNT-1和SNT-3中,用于突触外细胞形成的机制既有保留性,也有分歧性.
- 由Ca2+触发的快速和缓慢的神经递质释放通过不同的策略运作.
- 这项研究阐明了双传感器对神经递质释放的复杂调节.
更多相关视频
12:01Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals
Published on: October 1, 2014
9.2K
08:10Examination of Synaptic Vesicle Recycling Using FM Dyes During Evoked, Spontaneous, and Miniature Synaptic Activities
Published on: March 31, 2014
21.5K
相关概念视频
Fusion of Secretory Vesicles with the Plasma Membrane
16.5K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
16.5K
Overview of Secretory Vesicles
9.3K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.3K
SNAREs and Membrane Fusion
12.3K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
12.3K
Pinching-off of Coated Vesicles
4.0K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.0K
Vesicular Tubular Clusters
3.0K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
3.0K
Exocytosis
8.9K
Exocytosis is a process that releases molecules outside the cell. Like other bulk transport mechanisms, exocytosis requires energy.
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
8.9K
