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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Computed Tomography01:10

Computed Tomography

7.9K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.9K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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相关实验视频

Updated: Jan 11, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

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Vis-OCT Explorer:一种开源软件,用于可见光光学连贯性断层扫描数据处理.

Weijia Fan, Fengyuanshan Xu, Roman Kuranov

    bioRxiv : the preprint server for biology
    |November 19, 2025
    PubMed
    概括
    此摘要是机器生成的。

    我们开发了Vis-OCT Explorer,这是一个开源软件,用于可见光光学连贯断层扫描 (vis-OCT) 图像处理. 该工具提高了图像质量,并确保可重复的视网膜厚度测量,这对于临床应用至关重要.

    更多相关视频

    Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
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    相关实验视频

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    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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    Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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    In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
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    科学领域:

    • 眼科医生 眼科 眼科
    • 生物医学成像技术 生物医学成像技术
    • 光学工程是指光学工程.

    背景情况:

    • 可见光光学相干断层扫描 (vis-OCT) 提供了先进的视网膜可视化.
    • 高质量的视OCT图像重建需要专门的后处理技术,如平衡检测.
    • 一个标准化,开源的软件解决方案对于临床翻译vis-OCT技术至关重要.

    研究的目的:

    • 开发和评估Vis-OCT Explorer,这是一个基于Python的开源软件,用于处理Vis-OCT图像.
    • 实现先进的处理功能,包括双光谱仪平衡检测和基于STFT的分散补偿优化.
    • 评估软件在图像重建质量和视网膜厚度测量的可重复性方面的性能.

    主要方法:

    • 开发了Vis-OCT Explorer,这是一个模块化软件,具有标准的OCT处理 (k空间重新采样,分散补偿,FT).
    • 集成的独特功能:双光谱仪平衡检测和STFT,以优化分散补偿.
    • 通过使用质量指数评估重建质量,并通过对多个运营商和地点进行类内相关系数 (ICC) 分析评估测量可重复性.

    主要成果:

    • 平衡检测和基于STFT的分散补偿显著改善了重建的B扫描图像的质量指数.
    • 视网膜神经纤维层 (RNFL) 和质细胞内状层 (GCIPL) 厚度测量在87.5%的斑点中心图像中实现了高重复性 (ICC > 0.8).
    • 在所有视神经以头部为中心的图像中观察到RNFL厚度测量的出色重复性 (ICC > 0.8).

    结论:

    • 视OCT探索器为视OCT人类视网膜图像提供高质量的图像处理.
    • 该软件能够进行高度可重复的视网膜厚度测量,支持临床验证.
    • 这种工具对于促进多中心临床试验以确定vis-OCT的疗效至关重要.