在神经管形态发生过程中,TWIST1调节小长度,内细胞囊动力学和细胞-细胞结合
Derrick Thomas1, Brittany M Hufft-Martinez2, Zarna Lalwani1
1Department of Diagnostic and Biomedical Sciences, Center for Craniofacial Research, School of Dentistry, University of Texas Health Science Center at Houston, Texas, United States of America.
bioRxiv : the preprint server for biology
|November 19, 2025
概括
TWIST1 (Twist相关蛋白1) 通过影响乳毛形成,内细胞囊动力学和细胞-细胞结合,调节神经外皮形态. 它的缺失导致神经管异常发育和细胞失调.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞内核对胚胎发育至关重要,它调节了血重塑和纤维生殖.
- 已知TWIST1 (Twist相关蛋白1) 影响神经管闭合和神经细胞命运.
- TWIST1在神经管形态发生中的特定作用以前是未知的.
研究的目的:
- 为了研究TWIST1在神经外皮形态学中的功能.
- 了解TWIST1突变体中神经外皮异常背后的分子机制.
- 检查TWIST1在乳毛形成中的作用,粘附结合完整性和内细胞囊动力学.
主要方法:
- 用免疫光分析观察TWIST1局部化与细胞标记物.
- 在TWIST1淘汰胚胎中分析了毛长度和数量.
- 定量PCR和西斑用于评估基因和蛋白质表达水平.
- 检查内细胞囊泡积累和基因表达特征.
主要成果:
- 细胞质TWIST1局部化与β-catenin和内细胞调节剂 (LRP2,RAB11B) 在神经ectoderm的顶端表面.
- 淘汰TWIST1导致纤毛细胞的长度和数量减少.
- 在TWIST1突变体中观察到RAB11B和β-catenin的上调,无论是mRNA还是蛋白质水平.
- 观察到尾内细胞囊泡积累增加和内细胞基因表达变化,影响细胞-细胞结合点.
结论:
- TWIST1已被确立为神经外皮形态的调节者.
- TWIST1调节纤维生成,内细胞囊动力学和细胞-细胞结合完整性.
- 这些发现提供了关于神经管发育和相关缺陷的分子基础的见解.
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