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相关概念视频

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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相关实验视频

Updated: Jun 9, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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使用完整的基因组学/MGI测序平台在固体组织中进行高质量的染色质谱分析的精细ChIP-Seq协议.

Hayley Alloway1,2, Louisa Wiede3, Daniel Loos3

  • 1Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.

Current protocols
|November 19, 2025
PubMed
概括

这项研究为固体组织,特别是结肠直肠癌提供了一个优化的染色质免疫沉,其次是测序 (ChIP-seq) 协议. 这种精细的方法简化了组织处理,以便在体内可复制,灵敏地分析色素状态.

关键词:
在 ChIP-seq.在MGI中,MGI是MGI.表观遗传学是指表观遗传学.基因组 基因组 基因组测序的测序是指测序的测序.

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相关实验视频

Last Updated: Jun 9, 2026

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 癌症研究 癌症研究

背景情况:

  • 染色体免疫沉接着测序 (ChIP-seq) 对于理解基因调节和染色体状态至关重要.
  • 研究组织提供了一个比细胞培养更生理相关的模型来分析细胞异质性和空间组织.
  • 现有的ChIP-seq协议在应用于固体组织时面临挑战,原因是复杂性和低投入材料.

研究的目的:

  • 专门为固体组织开发和呈现一种优化的染色质免疫沉,其次是测序 (ChIP-seq) 协议.
  • 解决目前在组织分析中ChIP-seq方法的局限性,专注于结直肠癌.
  • 为了使与疾病相关的染色质状态在体内能够进行可复制,敏感和可扩展的分析.

主要方法:

  • 从冷样本制备组织的简化和高效程序.
  • 针对复杂组织矩阵的优化色素提取和免疫沉技术.
  • 简化图书馆建设和DNA纳米球的准备,用于高通量测序平台.

主要成果:

  • 该协议克服了与ChIP-seq. 组织处理相关的常见限制.
  • 实现了固体组织中染色质状态的高度可重现和敏感的分析.
  • 在体内对疾病相关的染色质研究中证明了可扩展性.

结论:

  • 优化的ChIP-seq协议促进了固体组织中强大的染色质分析,包括癌症研究.
  • 这种精细的方法增强了对体内染色质动态和表观遗传调节的研究.
  • 该协议为研究本地组织环境中的疾病机制提供了有价值的工具.