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尼提丁化物通过向BUB1来抑制结直肠癌:来自分子动力学模拟,空间转录组学和单细胞RNA测序的机械洞察力

Ke-Jun Wu¹, Jin-Cheng Li², Li-Min Liu³

¹Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, 530021, China.

BMC gastroenterology

|November 19, 2025

概括

尼提丁化物 (NC) 通过降低BUB1表达的调节,对结直肠癌 (CRC) 产生抗瘤作用. 这种天然类化合物破坏RAD21-BUB1轴,抑制瘤生长并抑制免疫微环境.

科学领域:

在瘤学瘤学.
药理学药理学是指药理学的学科.
分子生物学分子生物学

背景情况:

结肠直肠癌 (CRC) 是一个重大的临床挑战,因为治疗疗效有限.
尼提丁化物 (NC),一种天然化物,显示出潜在的抗瘤活性,但其在CRC中的精确机制需要阐明.

研究的目的:

研究尼提丁化物 (NC) 在结直肠癌 (CRC) 中的抗瘤作用和潜在机制.
探索NC作为治疗CRC治疗的治疗策略的潜力.

主要方法:

用细胞活力测试 (细胞计数套件-8) 和异种移植小鼠模型来评估NC的疗效.
包括RNA测序,单细胞RNA测序和空间转录组学在内的多组学方法被用于识别关键分子标.
进行了分子动力学模拟和免疫相关性分析,以阐明NC,BUB1,RAD21和瘤免疫微环境之间的相互作用.

主要成果:

在体内,NC抑制了CRC细胞活力,降低了瘤生长,亡和血管生成.
转录组分析发现BUB1是核心下调基因,BUB1的高表达与CRC恶性瘤和免疫抑制有关.
证实NC与BUB1直接相互作用,减少其表达并破坏RAD21-BUB1轴,从而减轻免疫逃避.

结论:

尼提丁化物 (NC) 通过下调BUB1.1,具有显著的抗CRC作用.
通过NC破坏RAD21-BUB1轴对其抗瘤活性起着至关重要的作用.
在结直肠癌中,NC证明了克服BUB1-介导免疫抑制的潜力,提供了一个有前途的治疗途径.

关键词:

结肠直肠癌是一种癌症.免疫微观环境免疫微观环境尼提丁化物尼提丁化物在RAD21BUB1轴上单细胞测序是一种单细胞测序.空间转录组学空间转录组学

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