在体外成熟过程中,生理氧张力对人类积卵细胞复合物的代谢的影响:一项探索性研究
Ellen Anckaert1,2, Gamze Ates3, Arjen Liveyns2
1Research Group Genetics, Reproduction and Development (GRAD), Follicle Biology Laboratory (FOBI), Vrije Universiteit Brussel (VUB), Brussels, 1090, Belgium.
Journal of ovarian research
|November 19, 2025
概括
在体外成熟 (IVM) 培养过程中,生理氧水平 (5%) 在人类积卵细胞复合体 (COCs) 中改善能量代谢. 这一发现表明,优化氧气条件可以提高IVM对辅助生殖的有效性.
科学领域:
- 生殖生物学 生殖生物学
- 细胞的新陈代谢
- 辅助生殖技术 辅助生殖技术
背景情况:
- 试管成熟 (IVM) 是一种患者友好的辅助生殖技术,特别适用于患有PCOS的女性.
- 双相IVM协议显示出希望,但效果不如传统方法.
- 低于最佳的培养条件,如高氧,可能会损害卵细胞的能力.
研究的目的:
- 为了研究不同氧度对人类积卵细胞复合体 (COCs) 在IVM培养前的能量代谢的影响.
- 评估生理 (5%) 与大气 (20%) 氧如何影响COC代谢功能和卵细胞质量.
- 确定最佳的培养条件,以改善IVM结果.
主要方法:
- 从小毛囊中培养的人类COCs在5%或20%氧气下培养了24小时.
- 使用海马试验 (ATP生产,线粒体呼吸) 评估了代谢功能.
- 分析了COC的ATP水平,消耗的介质组成和卵细胞的线粒体/抗氧化标志物.
主要成果:
- 在5%氧气培养的COC显示出较高的ATP度,线粒体呼吸减少.
- 葡萄糖摄入量保持稳定,而乳酸盐产量增加了5%以下的氧气.
- 线粒体潜力和抗氧化标志物不受氧气度的影响.
结论:
- 在IVM前,生理氧 (5%) 提高了人类COCs的代谢效率.
- 这些发现表明,在低氧条件下,人们正在转向替代能源基质 (例如脂肪酸).
- 重新评估和优化IVM培养氧气水平以模仿体内条件至关重要,以提高卵细胞的能力.
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