相关实验视频
Updated: Jan 11, 2026
05:19
Surgical Models of Gastroesophageal Reflux with Mice
Published on: August 25, 2015
10.7K
Smurf1通过调节Axin2依赖的Wnt信号通路来促进胃癌的进展
Jinling Yu1, Jiachen Jing1, Zhen Feng2
1Department of Gastroenterology, Xu Hui District Center Hospital, Xuhui District, Shanghai, 200031, China.
Scientific reports
|November 19, 2025
概括
特定于SMAD的E3泛素蛋白联酶1 (Smurf1) 在胃癌 (GC) 中充当瘤基因. 升级的Smurf1通过降解Axin2,激活Wnt/β-catenin信号,并恶化患者的生存,促进GC的进展.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 生物化学 生物化学
背景情况:
- 特定于SMAD的E3泛素蛋白联酶1 (Smurf1) 针对蛋白质进行降解,影响生物过程.
- Smurf1越来越多地被认为是各种人类癌症的瘤基因,包括胃癌 (GC).
研究的目的:
- 调查Smurf1在调节GC进展中的作用.
- 阐明GC中Smurf1的潜在分子机制.
主要方法:
- 通过qRT-PCR对公开可用的数据集和GC组织中的Smurf1表达的分析.
- 通过细胞培养和小鼠异种移植模型对Smurf1在GC细胞中的生物作用进行了体外和体内评估.
- 研究Smurf1与轴抑制蛋白2 (Axin2) 的相互作用及其对Wnt/β-catenin信号传递的影响.
主要成果:
- 与正常组织相比,GC组织中的Smurf1表达显著增加,与较差的无病生存率相关.
- 在Smurf1的过度表达增强了GC细胞的生长,增殖和侵入,在体外和体内.
- Smurf1与Axin2直接相互作用,通过无化和降解降低其稳定性,导致Wnt/β-catenin通路的激活.
- 使用IWR-1抑制Wnt通路,抵消了Smurf1驱动的GC细胞增殖和入侵.
结论:
- 升级的Smurf1是胃癌进展的驱动因素.
- Smurf1通过促进Axin2降解和激活Wnt/β-catenin信号通路来促进GC的进展.
- 准Smurf1或Wnt通路可能为胃癌提供治疗策略.
相关概念视频
02:54Canonical Wnt Signaling Pathway
10.4K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.4K
02:23Abnormal Proliferation
5.1K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
03:03mTOR Signaling and Cancer Progression
4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.6K