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Updated: Jan 11, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

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可编程的无特异性基因分析系统通过基于双循环的滚动循环放大与高效的CRISPR/Cas12a生物传感策略

Chunfeng Feng1,2, Yunxia Wang3, Chang Liu1,2

  • 1Department of Clinical Laboratory, The Second Affiliated Hospital (Xinqiao Hospital), Army Medical University, Chongqing, 400037, China.

Scientific reports
|November 19, 2025
PubMed
概括

一个新的CRISPR/Cas12a系统使用nicking内核酶辅助的目标回收和指数滚动圆放大用于超敏感DNA检测. 这种方法提供了高准确性的癌症查,避免了非特异性放大,以改善诊断.

关键词:
这就是CRISPR/Cas12a.指数式滚动圆的放大器.尼克的内核酶被断了.没有-没有特定的放大.目标是回收回收.

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