相关实验视频

Updated: Jan 6, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Published on: November 8, 2015

在高剂量治疗中测量甲状腺素的新型LC-MS/MS方法:与商业EMIT和EIA免疫试验进行比较研究

Agnieszka Czajkowska¹, Aleksandra Mikulska², Martyna Poniewierska³

¹Therapeutic Drug Monitoring, Clinical Pharmacokinetics and Toxicology Laboratory Unit, Department of Clinical Biochemistry, The Children's Memorial Health Institute, Warszawa, 04-730, Poland.

Pharmacological reports : PR

|November 19, 2025

概括

在儿童中,高剂量甲状腺素监测至关重要. 液体染色学-双重质谱法 (LC-MS/MS) 与免疫试验相比,提供更高的准确性,防止毒性并优化治疗.

科学领域:

儿科瘤学儿科瘤学
临床化学临床化学
药理动力学药理动力学

背景情况:

高剂量的甲状腺素 (HDMTX) 对儿科癌症至关重要.
由于MTX的治疗指数狭窄,治疗药物监测 (TDM) 非常重要.
精确的MTX测量指导leucovorin救援并防止毒性.

研究的目的:

将EMIT和EIA免疫试验的分析性能与HDMTX的LC-MS/MS进行比较.
评估儿科瘤患者的试验准确性.
识别常规监控方法中的潜在偏差.

主要方法:

开发并验证了一种液体染色学-并联质谱法 (LC-MS/MS) 试验.
分析了接受HDMTX的儿科患者的临床样本.
将LC-MS/MS与EMIT和EIA免疫试验的结果进行比较.

主要成果:

LC-MS/MS测定符合ICH M10指南,具有出色的线性和灵敏度.
在所有方法之间观察到强烈的相关性 (r > 0.93).
免疫试验显示,由于与MTX代谢物 (DAMPA,7-OH-MTX) 的交叉反应,可能会高估MTX水平.

结论:

LC-MS/MS为HDMTX监控提供了卓越的准确性.
免疫检测,虽然可访问,可能会高估MTX水平,影响leucovorin救援.
选择适当的测定对于优化HDMTX治疗和患者安全至关重要.

关键词:

对于EIA来说,这是一个很好的选择.艾米特 (EMIT) 是一个国际组织.这是LC-MS/MS.甲基甲酸二醇的使用方法儿科瘤学儿科瘤学治疗药物监测治疗药物监测

更多相关视频

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...