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在宫癌中对长非编码RNA的分子和生物信息学分析

Shalu Ranga¹, Ritu Yadav², Meenakshi B Chauhan³

¹Department of Genetics, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.

|November 19, 2025

概括

在宫癌 (CC) 中,LINC00518基因促进体被低甲基化,这表明其瘤性作用和作为生物标记物的潜力. MAFG-AS1甲基化在CC组织中没有显著差异.

科学领域:

分子瘤学分子瘤学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
癌症生物标志物癌症生物标志物

背景情况:

长非编码RNAs (lncRNAs) 在宫癌 (CC) 中发挥调节作用.
评估特定lncRNAs的基因表达和甲基化可以揭示CC的治疗点和生物标志物.

研究的目的:

在宫癌患者中研究LINC00518和MAFG-AS1的基因表达和促进物甲基化状态.
探索LINC00518和MAFG-AS1甲基化与HPV感染,临床病理因素和患者存活率的关联.

主要方法:

定量实时PCR (qRT-PCR) 和甲基化特异PCR (MS-PCR) 在81名CC患者身上进行.
使用GEPIA和TANRIC数据库进行基因表达和生存分析的生物信息分析.
用RNAinter数据库来识别 lncRNAs 的潜在交互伙伴.

主要成果:

LINC00518促进物低甲基化与其在CC中的致癌行为有显著的相关性 (p < 0.05).
在癌症和正常组织之间没有发现MAFG-AS1甲基化的显著差异.
LINC00518甲基化状态与年龄,组织学亚型和分化程度有显著的相关性 (p < 0.001).
总体存活率分析表明LINC00518具有显著的预后值 (p < 0.05).

结论:

LINC00518 低甲基化可能会导致其失调,并在宫癌中发挥瘤作用.
LINC00518具有作为预测CC严重程度和预后的临床生物标志物的潜力.
MAFG-AS1甲基化状态不是宫癌进展的重要因素.

关键词:

宫癌是发生在宫癌的原因之一.这是一个HPVHPVHPV.低甲基化是一种低甲基化.卡普兰 - 梅尔曲线长的非编码RNA是什么?总体生存率总体生存率预测预后预测预测

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