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雪格拉菌体Sf11的尾部结构和宿主附着机制

Sundharraman Subramanian¹, John A Dover¹, Kristin N Parent¹

¹Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, USA.

Journal of virology

|November 19, 2025

概括

我们确定了Shigella吸虫的冷EM结构,揭示了它的囊和尾部蛋白质. 尾尖蛋白识别宿主LPS,为治疗菌体设计提供了潜力.

科学领域:

结构生物学结构生物学
病毒学病毒学
菌体研究研究菌体研究

背景情况:

对于Shigella菌体的结构数据有限,之前的研究集中在非收缩性菌体和单个收缩性肌菌体上.
了解Shigella菌体结构对于它们在治疗和工业中的潜在应用至关重要.

研究的目的:

通过冷电子显微镜 (cryo-EM) 确定一株西格拉菌体 (菌体Sf11) 的结构.
识别和建模菌体的结构部件,特别是它的尾巴.
为了研究尾尖蛋白在宿主识别中的功能.

主要方法:

使用冷电子显微镜 (cryo-EM) 可视化了菌体结构.
质谱法被用来识别 virion 中的蛋白质.
对大多数菌体蛋白质生成了原子模型.

主要成果:

冷EM结构揭示了囊体和尾部件的详细可视化,包括尾管蛋白 (TTP),距离尾部蛋白 (DTP),基板枢纽蛋白 (BHUB1和BHUB2) 和带测量蛋白 (TMP).
鉴定出六种类似于Sf6类和P22类菌体中的三元体尾尖蛋白来装饰尾部.
宿主范围研究证实,这些尾尖蛋白识别了宿主脂多糖 (LPS) 上的O-抗原,类似于相关的菌体.

结论:

体Sf11拥有一个尾部适配器域,可以用酶性,P22类尾尖蛋白来装饰它的尾部.
这种结构特征增强了我们对Shigella菌体之间的进化关系的理解.
这些发现表明,这些菌体结构的潜在应用是作为蛋白质支架,用于开发用于治疗或工业用途的工程菌体.

关键词:

石格拉菌体的菌体细菌菌体是一种菌体.化-EM结构的结构.菌体进入菌体的入口吸水体 (Siphophage) 是一种吸水体.尾巴尾巴尾巴尾巴尾巴在线

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