从星 (Platichthys stellatus) 中分离的Streptococcus parauberis的基因组和表型特征:第一次全基因组分析,致病性评估和抗菌素耐药性分析
Ji-Hwan Park1, Jeong-Won Byun2, Si-Ah Kim1
1School of Marine and Fisheries Life Science, Pukyong National University, Busan, Republic of Korea.
Journal of fish diseases
|November 19, 2025
概括
星状鱼水产养殖中Streptococcus parauberis会导致大量的死亡. 这项研究将特定的血清型和抗生素耐药性基因与疾病爆发联系起来,为更好的管理策略提供信息.
科学领域:
- 水生动物卫生 水生动物卫生
- 细菌学 细菌学是一门学科.
- 海洋水产养殖的海洋水产养殖
背景情况:
- 杆菌 (Streptococcus parauberis) 是一种影响海洋水产养殖的重要病原体.
- 之前的报道表明,它存在于韩国Platichthys stellatus (星) 养殖场.
- 大规模死亡事件需要识别致病原体.
研究的目的:
- 为了确定细菌病原体,负责群众死亡的明星.
- 描述分离物,包括血清型,毒性因子和抗生素耐药性概况.
- 调查S. parauberis特征与疾病爆发之间的相关性.
主要方法:
- 每月对疾病进行监测,并从受影响的农场中分离细菌.
- 使用SPA原料识别S. parauberis.
- 通过内注射在明星鱼中进行致病性测试.
- 全基因组测序和分离物的比较分析.
- 抗生素耐药性和毒性基因的PCR检测.
主要成果:
- 确定了31个S. parauberis分离物,所有分类为Ia血清型,表现出γ-hemolysis.
- 菌株AD112410在明星鱼中表现出剂量依赖的致病性.
- 所有的分离物都具有毒性基因 (gapC,hasC) 和抗性基因.
- 对四环素和氧三环素的耐药性是普遍的;对红色素和克林达胺素的耐药性随着 erm (B) 的存在而变化.
- 在C农场的分离物体中,erm(B) 基因不存在.
结论:
- 证实S. parauberis是星状鱼群中大量死亡的致病原体.
- 在S. parauberis血清型Ia,抗生素耐药性基因 (tet(S), erm(B) 和抗生素敏感性模式之间存在相关性.
- 这些发现为管理海洋水产养殖中的S. parauberis感染提供了关键的见解.
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