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Updated: Jan 11, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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优化了培养和提取菌分离物中DNA的协议,这些分离物与松树中的棕色斑点针病有关

Temitope Ruth Folorunso1, Gabriel Silva1, Marilis E Girón2

  • 1College of Forestry, Wildlife, and Environment, Auburn University, Auburn, Alabama, United States of America.

PloS one
|November 19, 2025
PubMed
概括

萨博劳德德克斯特培养和CTAB PVP DNA提取为高质量的真菌DNA提供了强大的管道. 这种方法优化了生长和纯度,用于研究Pinus物种的棕斑针病原体.

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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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